Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource
| Autor: | Jessica Dantzer, Sara Dirscherl, Xiaoman Li, Jonathan Nowacki, Scott Cross, Sean D. Mooney, Kenneth Cornetta, Brandon Peters, Mary C. Dinauer |
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| Rok vydání: | 2008 |
| Předmět: |
Virus Integration
Computational biology Biology Genome Polymerase Chain Reaction Article Software Resource (project management) Databases Genetic Genetics Animals Humans Molecular Biology Gene Internet Integrases business.industry Research Chromosome Mapping Genetic Therapy Automation Retroviridae Molecular Medicine The Internet Web resource business |
| Zdroj: | Gene therapy. 15(18) |
| ISSN: | 1476-5462 |
| Popis: | Research in gene therapy involving genome-integrating vectors now often includes analysis of vector integration sites across the genome using methods such as ligation-mediated PCR (LM-PCR) or linear amplification-mediated PCR (LAM-PCR). To help researchers analyze these sites and the functions of nearby genes, we have developed SeqMap (http://seqmap.compbio.iupui.edu/) a secure, web-based comprehensive vector integration site management tool that automatically analyzes and annotates large numbers of vector integration sites derived from LM-PCR experiments in human and model organisms upon a common genome database. We believe the use of this resource will enable better reproducibility and understanding of this important data. |
| Databáze: | OpenAIRE |
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