Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
| Autor: | Sumio Iijima, Katsutoshi Kokubun, Kiyotaka Shiba, Masako Yudasaka, Sachiko Matsumura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Medicine (General)
Time Factors Pharmaceutical Science Peptide peptide aptamer 02 engineering and technology 01 natural sciences Dexamethasone Mice Drug Delivery Systems International Journal of Nanomedicine Drug Discovery drug carrier Original Research Titanium chemistry.chemical_classification Chemistry carbon nanomaterial General Medicine 021001 nanoscience & nanotechnology Drug delivery 0210 nano-technology Drug carrier Oxidation-Reduction Cell Survival Surface Properties Aptamer Biophysics Bioengineering Nanotechnology 010402 general chemistry Cell Line Biomaterials Adsorption carbon nanohorn R5-920 Animals Amino Acid Sequence Bone regeneration Nanotubes Carbon Organic Chemistry Quartz crystal microbalance Alkaline Phosphatase 0104 chemical sciences Drug Liberation Immobilized Proteins drug delivery Nanocarriers Peptides |
| Zdroj: | International Journal of Nanomedicine, Vol Volume 13, Pp 1643-1652 (2018) International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Katsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan; 4Graduate School of Science and Technology, Meijo University, Nagoya, Japan Introduction: Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs.Materials and methods: We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti.Results: Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period.Conclusion: This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration. Keywords: drug carrier, drug delivery, carbon nanomaterial, carbon nanohorn, peptide aptamer |
| Databáze: | OpenAIRE |
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