Inhibition of farnesyl pyrophosphate synthase improves pressure overload induced chronic cardiac remodeling
| Autor: | Jie Ding, Chen-Ze Zhao, Yun Mou, Dongpu Dai, Jian Yang, Xu-Ming Zhao, Huan-Dong Wu, Shen-Jiang Hu, Bin Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine RHOA Farnesyl pyrophosphate Down-Regulation Mevalonic Acid Blood Pressure Cardiomegaly Mice Transgenic Mevalonic acid 030204 cardiovascular system & hematology Pharmacology Article Sarcoplasmic Reticulum Calcium-Transporting ATPases Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fibrosis Renin–angiotensin system medicine Animals Aorta Abdominal Ventricular remodeling Monomeric GTP-Binding Proteins Mitogen-Activated Protein Kinase 1 Pressure overload Mitogen-Activated Protein Kinase 3 Multidisciplinary Ventricular Remodeling biology business.industry Myocardium Geranyltranstransferase medicine.disease 030104 developmental biology chemistry ras Proteins biology.protein Calcium RNA Interference Mevalonate pathway rhoA GTP-Binding Protein business |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep39186 |
| Popis: | Farnesyl pyrophosphate synthase (FPPS) is a key enzyme in the mevalonate pathway. In our previous studies, we find that inhibition of FPPS attenuates angiotensin II-induced cardiac hypertrophy and fibrosis by suppressing RhoA while FPPS and Ras are up-regulated in pressure overload rats. In this study, we evaluate the effects and mechanisms of FPPS inhibition in pressure overload mice. Male FPPS-small interfering RNA (SiRNA) transgenic (Tg) mice and non-transgenic littermate control (NLC) were randomly divided into suprarenal abdominal aortic constriction (AAC) group and sham operation group. 12 weeks following AAC, mice were sacrificed by cervical dislocation. Histological and echocardiographic assessments showed that inhibition of FPPS improved chronic cardiac remodeling which was induced by AAC. The reductions of Ras farnesylation and GTP-Ras, as well as their downstream extracellular signal-related kinases 1/2 (ERK1/2) expression were observed in the heart of Tg-AAC mice compared with NLC-AAC mice, along with the reduction of fetal gene expression. We provide here important experimental evidence that inhibition of FPPS improves AAC induced chronic cardiac remodeling and fibrosis by the reduction of farnesylated Ras and the downregulation of Ras-ERK1/2 pathway. |
| Databáze: | OpenAIRE |
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