Cell‐Templated Silica Microparticles with Supported Lipid Bilayers as Artificial Antigen‐Presenting Cells for T Cell Activation
| Autor: | Joshua A. Gustafson, Bryan Kaehr, Suzie H. Pun, Ian I. Cardle, Ashley Wilson, Michael C. Jensen, Yu-Shen Lin, Caleb R. Perez, Brynn R Olden |
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| Rok vydání: | 2018 |
| Předmět: |
CD4-Positive T-Lymphocytes
Erythrocytes T cell Lipid Bilayers Cell Cell Culture Techniques Biomedical Engineering Antigen-Presenting Cells Pharmaceutical Science 02 engineering and technology CD8-Positive T-Lymphocytes Lymphocyte Activation 010402 general chemistry Proof of Concept Study 01 natural sciences Antibodies Article Biomaterials HeLa Artificial antigen presenting cells Membrane fluidity medicine Humans Particle Size Lipid bilayer biology Chemistry Cell Differentiation Silicon Dioxide 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Membrane medicine.anatomical_structure Biophysics 0210 nano-technology CD8 HeLa Cells |
| Zdroj: | Advanced Healthcare Materials. 8:1801188 |
| ISSN: | 2192-2659 2192-2640 |
| DOI: | 10.1002/adhm.201801188 |
| Popis: | Biomaterial properties that modulate T cell activation, growth, and differentiation are of significant interest in the field of cellular immunotherapy manufacturing. In this work, we present a new platform technology that allows for the modulation of various activation particle design parameters important for polyclonal T cell activation. We successfully create artificial antigen presenting cells (aAPCs) using supported lipid bilayers on various cell-templated silica microparticles with defined membrane fluidity and stimulating antibody density. We use this panel of aAPCs to probe the importance of activation particle shape, size, membrane fluidity, and stimulation antibody density on T cell outgrowth and differentiation. All aAPC formulations were able to stimulate T cell growth, and preferentially promoted CD8(+) T cell growth over CD4(+) T cell growth when compared to commercially available pendant antibody-conjugated particles. T cells cultured with HeLa and red blood cell templated aAPCs had a less differentiated and less exhausted phenotype than those cultured with spherical aAPCs with matched membrane coatings when cultured for 14 days. These results support continued exploration of silica supported lipid bilayers as an artificial antigen presenting cell platform. |
| Databáze: | OpenAIRE |
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