Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes
| Autor: | Stefanie Van Trappen, Joris Mergaert, Jean Swings, Ilse Vandecandelaere |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Bacterial Geologic Sediments Genotype Sequence analysis Molecular Sequence Data Antarctic Regions Fresh Water DNA Ribosomal Flavobacterium Polymerase Chain Reaction Microbiology Species Specificity Phylogenetics RNA Ribosomal 16S Psychrophile Phylogeny Ecology Evolution Behavior and Systematics biology Phylogenetic tree Sequence Analysis DNA General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Flavobacteriaceae Cold Temperature Phenotype |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 55:769-772 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.03056-0 |
| Popis: | Taxonomic studies were performed on seven strains isolated from microbial mats in Antarctic lakes of the McMurdo Dry Valleys. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains are related to the genus Flavobacterium; sequence similarity values with their nearest phylogenetic neighbours ranged from 97·0 to 98·7 %. Results of DNA–DNA hybridization and comparison of repetitive extragenic palindromic DNA-PCR fingerprinting patterns revealed that these strains are members of two distinct species. Genotypic results, together with phenotypic characteristics, allowed these species to be differentiated from related recognized Flavobacterium species. The isolates are psychrophilic, Gram-negative, chemoheterotrophic, rod-shaped cells. Their whole-cell fatty acid profiles are similar and include C15 : 0, C15 : 0 anteiso, C15 : 0 iso, C15 : 1 ω6c, C16 : 0 iso, C16 : 0 iso 3-OH, C16 : 1 iso and summed feature 3 (which comprises C15 : 0 iso 2-OH and/or C16 : 1 ω7c) as the major fatty acid components. On the basis of these results, two novel species are proposed: Flavobacterium fryxellicola sp. nov., consisting of three strains with LMG 22022T (=CIP 108325T) as the type strain; and Flavobacterium psychrolimnae sp. nov., consisting of four strains with LMG 22018T (=CIP 108326T) as the type strain. DNA G+C contents of F. fryxellicola and F. psychrolimnae are 35·2–35·9 and 33·8–34·5 mol%, respectively. |
| Databáze: | OpenAIRE |
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