The Effects of Ascorbic Acid and Iron Co-Supplementation on the Proliferation of 3T3 Fibroblasts
| Autor: | Susan J. Peach, Clifford S. Collis, Anthony T. Diplock, Min Yang, Catherine Rice-Evans |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Antioxidant Cell division Iron medicine.medical_treatment Ascorbic Acid Biochemistry 3T3 cells Lipid peroxidation Mice chemistry.chemical_compound Malondialdehyde Internal medicine medicine Animals chemistry.chemical_classification Dose-Response Relationship Drug Cell growth Transferrin Drug Synergism 3T3 Cells General Medicine Ascorbic acid Culture Media Endocrinology medicine.anatomical_structure chemistry Lipid Peroxidation Cell Division |
| Zdroj: | Free Radical Research. 25:87-93 |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.3109/10715769609145658 |
| Popis: | Exposure of 3T3 fibroblasts to FeII reveals a concentration-dependent inhibition of cell proliferation compared to control cells, the apparent threshold for this iron-mediated effect being 5 microM FeII. The inhibition of cell proliferation was accompanied by an enhancement of total malondialdehyde (MDA) levels (as detected directly by hplc) in the cells at higher iron concentrations. The co-supplementation of FeII with varying concentrations of ascorbic acid over the range 5 microM to 240 microM had no significant effect on the threshold for iron toxicity or lipid peroxidation. These results show that there is neither a significant exacerbation of the pro-oxidant effect of FeII nor any protective effect of ascorbate when cultures of 3T3 mouse fibroblasts are exposed to co-supplementation regimes of iron with ascorbic acid. |
| Databáze: | OpenAIRE |
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