Involvement of Escherichia coli FIS protein in maintenance of bacteriophage Mu lysogeny by the repressor : control of early transcription and inhibition of transposition
| Autor: | Michael Chandler, I Poquet, Mireille Bétermier, Robert Alazard |
|---|---|
| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Transcription
Genetic Operon phage lambda Regulatory Sequences Nucleic Acid medicine.disease_cause Bacteriophage lac repressor Factor For Inversion Stimulation Protein Genetics 0303 health sciences biology Escherichia coli Proteins DNA-Binding Proteins [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Oligodeoxyribonucleotides Bacteriophage Mu Protein Binding Research Article binding-site Integration Host Factors ends dna-sequences Blotting Western Molecular Sequence Data cloning vehicles Repressor Microbiology Bacteriophage mu 03 medical and health sciences Lysogenic cycle Consensus Sequence expression Escherichia coli medicine RNA Messenger gene Lysogeny Molecular Biology Prophage 030304 developmental biology Base Sequence 030306 microbiology Genetic Complementation Test Gene Expression Regulation Bacterial biology.organism_classification Repressor Proteins integration host factor DNA Transposable Elements Mutagenesis Site-Directed Carrier Proteins strand-transfer-reaction |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 1993, 175 (12), pp.3798-3811. ⟨10.1128/jb.175.12.3798-3811.1993⟩ Scopus-Elsevier |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | International audience; The Escherichia coli FIS (factor for inversion stimulation) protein has been implicated in assisting bacteriophage Mu repressor, c, in maintaining the lysogenic state under certain conditions. In a fis strain, a temperature-inducible Mucts62 prophage is induced at lower temperatures than in a wild-type host (M. Bétermier, V. Lefrère, C. Koch, R. Alazard, and M. Chandler, Mol. Microbiol. 3:459-468, 1989). Increasing the prophage copy number rendered Mucts62 less sensitive to this effect of the fis mutation, which thus seems to depend critically on the level of repressor activity. The present study also provides evidence that FIS affects the control of Mu gene expression and transposition. As judged by the use of lac transcriptional fusions, repression of early transcription was reduced three- to fourfold in a fis background, and this could be compensated by an increase in cts62 gene copy number. c was also shown to inhibit Mu transposition two- to fourfold less strongly in a fis host. These modulatory effects, however, could not be correlated to sequence-specific binding of FIS to the Mu genome, in particular to the strong site previously identified on the left end. We therefore speculate that a more general function of FIS is responsible for the observed modulation of Mu lysogeny. |
| Databáze: | OpenAIRE |
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