Fused Late Endocytic Compartments and Immunostimulatory Capacity of Dendritic–Tumor Cell Hybridomas
| Autor: | Martina Bergant, Robert Zorec, Matjaž Jeras, Marko Kreft, Mateja Gabrijel |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
education.field_of_study
MHC class II Hybridomas biology Physiology Endocytic cycle Population Biophysics Priming (immunology) chemical and pharmacologic phenomena Dendritic Cells Cell Biology Molecular biology Endocytosis In vitro Cell biology Antigen biology.protein Humans Cytotoxic T cell Immunization Caco-2 Cells education Antigen-presenting cell |
| Zdroj: | Journal of Membrane Biology. 229:11-18 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/s00232-009-9171-7 |
| Popis: | Late endocytic compartments, containing MHC class II molecules in antigen presenting cells, fuse to each other in order to deliver antigens to these molecules. We have shown previously that fusion of late endocytic compartments takes place also in hybridomas. Therefore, we investigate here whether the level of fused late endocytic compartments affects the immunostimulatory capacity of hybridomas obtained by the electrofusion of dendritic and tumor cells. The level of fused late endocytic compartments in a single hybridoma cell was assessed and samples of electrofused cells were then cocultured with autologous T cells, resulting in the priming of naïve T cells. To test the immunostimulatory capacity of hybridoma cells, T-cell-induced cytotoxicity of tumor cells was assayed. The results demonstrate that in vitro cytotoxic T cell responses are enhanced if a higher percentage of fused late endocytic compartments is present in the cell population of electrofused hybridoma cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink