Joint participation of mitochondria and sarcoplasmic reticulum in the formation of tubular aggregates in gastrocnemius muscle of CK–/– mice
| Autor: | Guy Brochier, Ivan Zahradník, Marta Novotova, Michaela Pavlovicova, Renée Ventura-Clapier, Xavier Bigard |
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| Rok vydání: | 2002 |
| Předmět: |
Male
RNA Untranslated Histology Calcium-Transporting ATPases Mitochondrion Biology Microtubules Sarcoplasmic Reticulum Calcium-Transporting ATPases Pathology and Forensic Medicine Mice Ribonucleoproteins Small Cytoplasmic Gastrocnemius muscle Muscular Diseases medicine Animals Myocyte Muscle Skeletal Inner mitochondrial membrane Creatine Kinase Mice Knockout Endoplasmic reticulum Skeletal muscle Cell Biology General Medicine Immunogold labelling musculoskeletal system Immunohistochemistry Sarcoplasmic reticulum membrane Mitochondria Muscle Cell biology Disease Models Animal Microscopy Electron Sarcoplasmic Reticulum medicine.anatomical_structure RNA Long Noncoding |
| Zdroj: | European Journal of Cell Biology. 81:101-106 |
| ISSN: | 0171-9335 |
| DOI: | 10.1078/0171-9335-00230 |
| Popis: | Tubular aggregates are specific subcellular structures that appear in skeletal muscle fibres under different pathological conditions. The origin of the tubular aggregates is generally ascribed to proliferating membranes of sarcoplasmic reticulum. There are, however, histochemical indications for the presence of mitochondrial enzymes in tubular aggregates suggesting contribution of mitochondria to the genesis of tubular aggregates. In this study we used an immunocytochemical detection technique to assess participation of mitochondria and of sarcoplasmic reticulum in derivation of tubular aggregates. The fast skeletal muscle fibres (m. gastrocnemius) of mice bearing the double invalidation for both the mitochondrial and the cytosolic isoforms of creatine kinase (CK), an enzyme involved in energetics of muscle cells, were employed as a model muscle with tubular aggregates (Steeghs et al., Cell 89, 93-103, 1997). Immunogold labelling of the bc1 complex, a specific integral protein of the inner mitochondrial membrane, provided strong signals in both the mitochondria and tubular aggregates but not in other ultrastructural components of muscle fibres. A similar strong immunogold signal was obtained when labelling for SERCA1, a specific enzyme of the sarcoplasmic reticulum membrane, in regions of typical occurrence of the sarcoplasmic reticulum and in tubular aggregates. In double labelling experiments, we found simultaneous labelling of tubular aggregates with both the bc1 and SERCA1 antibodies. It is concluded, that in CK-/- mouse both the inner mitochondrial membrane and the membrane of the sarcoplasmic reticulum participate in the formation of tubular aggregates. |
| Databáze: | OpenAIRE |
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