Isolement d'un nouveau ceto-acide, produit a partir de ϵ-N -trimethyllysine par Neurospora crassa
| Autor: | Victor R. Villanueva, Edgar Lederer |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
chemistry.chemical_classification
biology Ion exchange Stereochemistry Metabolite Biophysics Hydrazone Cell Biology biology.organism_classification Biochemistry Neurospora crassa chemistry.chemical_compound chemistry Structural Biology Hydrogenolysis Ninhydrin Genetics lipids (amino acids peptides and proteins) Keto acid Molecular Biology Mycelium |
| Zdroj: | FEBS Letters. 52:308-311 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(75)80832-5 |
| Popis: | A new ϵ- N -trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa . The labelled compound produced from incubations in vivo and in vitro from ϵ- N -trimethyl ([ 14 C]H 3 )L-lysine has been identified as 2-keto-ϵ- N -trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazone back to ϵ- N -trimethyllysine by hydrogenolysis in a Parr bomb. Analyses on TLC and in four different ion exchange chromatographic systems show the appearance of a ninhydrin positive product having the same R f and the same retention time as ϵ- N -trimethyllysine; it contains more than 85% of the radioactivity of the 2,4-dinitrophenylhydrazone of the keto acid. |
| Databáze: | OpenAIRE |
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