Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment
| Autor: | Wonji Gu, Jinwoo Cheon, Heekyung Jeong, Jung Uk Lee, Jae Hyun Lee, Minsuk Kwak, Minji An, Yong Ho Kim, Young-wook Jun, Hyun Jung Lee |
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| Rok vydání: | 2019 |
| Předmět: |
Adherens junction formation
Nanoparticle Bioengineering Nanotechnology 02 engineering and technology single-cell perturbation biology Article Cell Line Polyethylene Glycols Micromanipulation Cell Line Tumor Humans General Materials Science Clickable Nanoscience & Nanotechnology Magnetite Nanoparticles Receptor cell surface microenvironment Fluorescent Dyes Tumor Chemistry Mechanical Engineering Optical Imaging Adherens Junctions General Chemistry Cadherins 021001 nanoscience & nanotechnology Condensed Matter Physics Actins Cell Microenvironment Cellular Microenvironment steric crowding Magnetic nanoparticles PEGylation Click chemistry Nanoparticles Click Chemistry 0210 nano-technology cell labeling |
| Zdroj: | Nano Lett Nano letters, vol 19, iss 6 |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/acs.nanolett.9b00891 |
| Popis: | Multifunctional magnetic nanoparticles have shown great promise as next-generation imaging and perturbation probes for deciphering molecular and cellular processes. As a consequence of multicomponent integration into a single nanosystem, pre-existing nanoprobes are typically large and show limited access to biological targets present in a crowded microenvironment. Here, we apply organic-phase surface PEGylation, click chemistry, and charge-based valency discrimination principles to develop compact, modular, and monovalent magnetofluorescent nanoparticles (MFNs). We show that MFNs exhibit highly efficient labeling to target receptors present in cells with a dense and thick glycocalyx layer. We use these MFNs to interrogate the E-cadherin-mediated adherens junction formation and F-actin polymerization in a three-dimensional space, demonstrating the utility as modular and versatile mechanogenetic probes in the most demanding single-cell perturbation applications. |
| Databáze: | OpenAIRE |
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