Keratinocyte-releasable factors increased the expression of MMP1 and MMP3 in co-cultured fibroblasts under both 2D and 3D culture conditions
| Autor: | Amy Lai, Aziz Ghahary, Min Li, Claudia Chavez-Munoz, Alireza Moeen Rezakhanlou |
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| Rok vydání: | 2009 |
| Předmět: |
Keratinocytes
Male MMP3 MMP1 Clinical Biochemistry Blotting Western Foreskin Matrix metalloproteinase Extracellular matrix Western blot medicine Humans RNA Messenger Fibroblast Molecular Biology Cells Cultured Oligonucleotide Array Sequence Analysis medicine.diagnostic_test Chemistry Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cell Biology General Medicine Fibroblasts Molecular biology Coculture Techniques Extracellular Matrix Blot medicine.anatomical_structure Matrix Metalloproteinase 3 Collagen Matrix Metalloproteinase 1 Keratinocyte Biomarkers |
| Zdroj: | Molecular and cellular biochemistry. 332(1-2) |
| ISSN: | 1573-4919 |
| Popis: | Matrix metalloproteinases (MMPs) are key elements in extracellular matrix (ECM) degradation and scar remodeling during the wound-healing process. Our previous data revealed that keratinocyte-releasable factors significantly increased the expression of fibroblast MMPs in monolayer-cultured fibroblasts. In this study, we analyzed the differences in the MMP expressions of fibroblasts in a three-dimensional fibroblast-populated collagen gel (3D FPCG) from that in a two-dimensional monolayer-cultured fibroblasts when both co-cultured with keratinocytes. Differential mRNA and protein expression of fibroblasts were examined by microarray, RT-PCR, and western blot. Our results showed that fibroblasts co-cultured with keratinocytes in a 3D FPCG expressed significantly higher MMP1 and MMP3 at the gene and protein levels. Due to the physiological advantages of a 3D FPCG model to a 2D system, we concluded that the 3D FPCG model may provide a better means of understanding the fibroblast-keratinocyte cross-talk during the wound-healing process. |
| Databáze: | OpenAIRE |
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