TrkB expression level correlates with metastatic properties of L1 mouse sarcoma cells cultured in non‐adhesive conditions

Autor:	J. Miloszewska, Małgorzata Przybyszewska, Paweł Swoboda, H. Trembacz, Monika Gos
Rok vydání:	2013
Předmět:	Lung Neoplasms Cell Survival Blotting Western Cell Biology Real-Time Polymerase Chain Reaction Mice Growth factor receptor Cell Movement Cell Line Tumor Gene expression Cell Adhesion medicine Animals Neoplasm Invasiveness Anoikis Neoplasm Metastasis Cell adhesion Tumor Stem Cell Assay Oligonucleotide Array Sequence Analysis Regulation of gene expression Mice Inbred BALB C Membrane Glycoproteins Sarcoma Original Articles Cell Biology General Medicine Protein-Tyrosine Kinases Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure Cell culture Cancer cell
Zdroj:	Cell Proliferation. 46:146-152
ISSN:	1365-2184 0960-7722
DOI:	10.1111/cpr.12015
Popis:	OBJECTIVES: Ability of a cell to survive without adhesion, and to overcome anoikis, is indispensable for malignant cell invasion and metastasis formation. It has previously been shown that TrkB ‐neutrophin growth factor receptor might be involved in suppression of apoptosis, induced by the lack of adhesion. The aim of our study was to analyse changes in expression of genes and proteins as well as in biological properties of cancer cells cultured without adhesion. A mouse sarcoma, stable, adherent L1 cell line, derived from a spontaneously arisen Balb/c mouse lung tumour, was established in vitro. MATERIALS AND METHODS: L1 cells resistant to anoikis were established by culture of L1 cells without adhesion, followed by selection of clones with elevated expression levels of TrkB protein. Biological characteristics of the cells were studied by migration/invasion tests and colony forming assay. Gene expression analysis was performed by with the aid of cDNA Gene Expression Array and Real‐Time PCR. In vivo experiments were conducted in syngeneic Balb/c mice. RESULTS: Significant changes in gene expression, including higher expression level of TrkB, were found in cells that were able to survive without adhesion. Selected TrkB‐expressing clones were found to have higher clonogenicity and invasive potential, formed more colonies in mouse lungs, and induced larger tumours, when injected subcutaneously into Balb/c mice. CONCLUSION: Lack of adhesion induced significant changes in the cancer cells’ behaviour, which may result from alterations in gene and protein expression levels, including changes in anoikis‐connected protein – TrkB.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7ff9117126b01593de3d8b159f7eaee https://doi.org/10.1111/cpr.12015 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.