Histidine racemization in the synthesis of an analog of the lutenizing hormone releasing factor
| Autor: | Björn Ekberg, Rüdiger Pipkorn |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Stereochemistry Stereoisomerism L-Amino Acid Oxidase Biochemistry High-performance liquid chromatography Peptide Fragments Gonadotropin-Releasing Hormone chemistry.chemical_compound Enzyme chemistry Peptide synthesis Histidine Indicators and Reagents Amino Acid Oxidoreductases Amino Acid Sequence Polystyrene Lhrh analog Carbonyldiimidazole Racemization Chromatography High Pressure Liquid |
| Zdroj: | International Journal of Peptide and Protein Research. 27:583-588 |
| ISSN: | 0367-8377 |
| DOI: | 10.1111/j.1399-3011.1986.tb01053.x |
| Popis: | To investigate histidine racemization in the synthesis of a LHRH analog, (D-Trp)6-LHRH2-10 was built up by stepwise elongation of the sequence 3-10 using the solid phase technique on a 1% cross-linked chloromethyl polystyrene. For the whole synthesis the tert.-butyloxycarbonyl (BOC) group was used for temporary N-terminal protection. To protect the pi-nitrogen in histidine the benzyl-oxymethyl (BOM) group was utilized. The condensation position in the (D-Trp)6-LHRH analog was chosen so as to be able to investigate the racemization of histidine. We coupled BOC-His(BOM) with the (D-Trp)6-LHRH3-10 fragment using three different activating agents, mixed anhydride, carbonyldiimidazole (1 equiv.)/1-hydroxybenzotriazole (2 equiv.) and dicyclohexylcarbodiimide/1-hydroxybenzotriazole. The racemization was investigated by enzymatic digestion and by HPLC. For HPLC, (D-His(BOM)2-(D-Trp)6-LHRH2-10 was also synthesized. It could be proved that practically no racemization occurs during the actual peptide synthesis. The small amount (1%) of D-histidine found is due to racemization in the synthesis of BOC-His(BOM). |
| Databáze: | OpenAIRE |
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