Short-term dietary copper deficiency does not inhibit angiogenesis in tumours implanted in striated muscle
| Autor: | D. M. Ackermann, Jack T. Saari, Mwr Reed, Olson, Dale A. Schuschke, Frederick N. Miller |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Pathology Angiogenesis Chondrosarcoma Weanling chemistry.chemical_element Biology Microcirculation Rats Sprague-Dawley Neoplasms Internal medicine medicine Animals Neovascularization Pathologic Muscles Albumin medicine.disease Copper Rats Endocrinology Liver Oncology chemistry Cremaster muscle Copper deficiency Neoplasm Transplantation Intravital microscopy Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | The effect of dietary copper deficiency on tumour growth, neovascularisation and microvascular integrity was studied in the rat cremaster muscle. Male, weanling Sprague-Dawley rats were fed purified diets which were copper deficient (< 0.5 micrograms g-1 of diet) or copper adequate (5 micrograms g-1 of diet). Seven days after initiation of diets, a chondrosarcoma was implanted in the cremaster muscle of each rat. Five, 10 or 20 days after tumour implantation, rats were anesthetised and their cremasters prepared for observation by intravital microscopy. Intraarterial injection of fluorescein isothiocyanate-conjugated albumin and subsequent observation of fluorescence in the perivascular space indicated no difference in microvascular albumin leakage between the tumour vasculature of copper deficient and copper adequate rats. Neither tumour growth (assessed by wet weight), vascular density (assessed by light microscopy), nor any ultrastructural characteristics of the tumour or its vasculature (assessed by electron microscopy) were affected by copper deficiency. In view of findings by others which indicate changes in tumour characteristics with copper deficiency, we conclude that the copper dependency of tumour growth and vascularisation is a function of the type of tumour, the host tissue, or the conditions of copper depletion. |
| Databáze: | OpenAIRE |
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