Dopamine as a Novel Antioxidative Agent for Rat Vascular Smooth Muscle Cells Through Dopamine D 1 -Like Receptors
| Autor: | Mieko Minami, Junichi Yoshikawa, Hiroaki Kano, Masakazu Kohno, Kenichi Yasunari |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Dopamine Antioxidants Muscle Smooth Vascular chemistry.chemical_compound Cell Movement Physiology (medical) Internal medicine Phospholipase D medicine Animals Calphostin Rats Wistar Receptor Cells Cultured Protein Kinase C Protein kinase C Platelet-Derived Growth Factor biology Receptors Dopamine D1 Benzazepines Oligonucleotides Antisense Cyclic AMP-Dependent Protein Kinases Immunohistochemistry H-89 Rats Oxidative Stress Endocrinology chemistry Dopamine receptor Dopamine Agonists biology.protein Dopamine Antagonists Cardiology and Cardiovascular Medicine Platelet-derived growth factor receptor medicine.drug |
| Zdroj: | Circulation. 101:2302-2308 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/01.cir.101.19.2302 |
| Popis: | Background —To elucidate the roles of vascular D 1 -like receptors in atherosclerosis, the effects of the specific D 1 -like agonists on platelet-derived growth factor (PDGF)-BB–mediated oxidative stress in vascular smooth muscle cells (VSMCs) were studied. Methods and Results —Immunohistochemical studies demonstrated the coexistence of D 1A and D 1B dopamine receptors in VSMCs. Western blotting revealed a band of ≈70 kDa for D 1A and D 1B dopamine receptors. VSMCs stimulated by PDGF-BB exhibited increased oxidative stress directly measured by flow cytometry. These effects were prevented by dopamine, SKF 38393, or YM 435, and this prevention was reversed by Sch 23390. These effects were blocked by a specific protein kinase A (PKA) inhibitor, N -(2-[ p -bromocinnamylamino]ethyl)-5-isoquinolinesulfonamide (H 89). The PDGF-BB–mediated increase in oxidative stress of VSMCs was significantly suppressed by the indirect phospholipase D (PLD) inhibitor suramin or the specific protein kinase C (PKC) inhibitor calphostin C. Both antisense but neither sense nor scrambled oligonucleotides to D 1A and D 1B receptors inhibited dopamine-induced suppression of increase in oxidative stress of VSMCs induced by PDGF-BB. Conclusions —These findings suggest that vascular D 1 -like receptors (D 1A and D 1B receptors) inhibit any increase in oxidative stress of VSMCs, possibly through activation of PKA and suppression of PLD and PKC. |
| Databáze: | OpenAIRE |
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