Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Autor: Archana Vijaya Kumar Vijaya Kumar, Carmen Donate, Beat A. Imhof, Thomas Matthes
Rok vydání: 2018
Předmět:
Heterografts/transplantation
0301 basic medicine
Cancer Research
General Chemical Engineering
Xenotransplantation
medicine.medical_treatment
Transplantation
Heterologous
Cell
Antineoplastic Agents
Lymphoma
Mantle-Cell
Mice
SCID
ddc:616.07
General Biochemistry
Genetics and Molecular Biology
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
Cell Line
Tumor
medicine
Animals
Humans
B-Lymphocytes/metabolism
Lymphoma
Mantle-Cell/surgery
B cell
ddc:616
B-Lymphocytes
General Immunology and Microbiology
business.industry
General Neuroscience
Antineoplastic Agents/therapeutic use
medicine.disease
Xenograft Model Antitumor Assays
Lymphoma
Transplantation
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cancer research
Heterografts
Mantle cell lymphoma
business
Homing (hematopoietic)
Zdroj: Journal of Visualized Experiments, No 133 (2018)
ISSN: 1940-087X
DOI: 10.3791/56023
Popis: B lymphocytes are key players in immune cell circulation and they mainly home to and reside in lymphoid organs. While normal B cells only proliferate when stimulated by T lymphocytes, oncogenic B cells survive and expand autonomously in undefined organ niches. Mantle cell lymphoma (MCL) is one such B cell disorder, where the median survival rate of patients is 4 - 5 years. This calls for the need of effective mechanisms by which the homing and engraftment of these cells are blocked in order to increase the survival and longevity of patients. Therefore, the effort to develop a xenograft mouse model to study the efficacy of MCL therapeutics by blocking the homing mechanism in vivo is of utmost importance. Development of animal recipients for human cell xenotransplantation to test early stage drugs have long been pursued, as relevant preclinical mouse models are crucial to screen new therapeutic agents. This animal model is developed to avoid human graft rejection and to establish a model for human diseases, and it may be an extremely useful tool to study disease progression of different lymphoma types and to perform preclinical testing of candidate drugs for hematologic malignancies, like MCL. We established a xenograft mouse model that will serve as an excellent resource to study and develop novel therapeutic approaches for MCL.
Databáze: OpenAIRE
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