BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis
| Autor: | Wenfan Liu, Bogdan A. Stoica, Geping Wang, Simone Di Giovanni, Alexander G. Yakovlev, Alan I. Faden |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Cell Survival DNA damage Immunoblotting Apoptosis Chromosomal translocation Transfection Biochemistry Cytosol Cell Line Tumor Proto-Oncogene Proteins medicine Humans Staurosporine RNA Messenger Molecular Biology Caspase Etoposide bcl-2-Associated X Protein Caspase 8 Microscopy Confocal Cell Death biology Caspase 3 Reverse Transcriptase Polymerase Chain Reaction Cytochrome c Intrinsic apoptosis Caspase 2 Cytochromes c Cell Biology Caspase 9 Cell biology Enzyme Activation Protein Transport Microscopy Fluorescence Proto-Oncogene Proteins c-bcl-2 Caspases biology.protein Cancer research RNA Interference Tumor Suppressor Protein p53 DNA Damage medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 279:28367-28374 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m313526200 |
| Popis: | Cellular stress leads to DNA damage and activation of the intrinsic apoptotic pathway in which translocation of mitochondrial cytochrome c to the cytosol plays a critical role. Previous studies have suggested alternative mechanisms responsible for this process. We examined initiation mechanisms of the intrinsic apoptotic pathway using human neuroblastoma and breast cancer cells. Results indicated that translocation of cytochrome c does not require prior activation of caspases but rather depends on activation of specific BCL-2 family members, depending upon the type of death signal. Thus, DNA damage-induced apoptosis requires new protein synthesis, accumulation of p53 tumor suppressor protein, and p53-dependent induction of BOK and NOXA genes, while a role for BAX in this pathway is not essential. In contrast, apoptosis induced by staurosporine does not require protein synthesis but is characterized by translocation of BAX. Based on these findings, we propose a model of the intrinsic apoptotic cascade induced by DNA damage where proapoptotic BOK substitutes for a function of BAX. |
| Databáze: | OpenAIRE |
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