Parallel study of transient dosing of antibiotics in a microfluidic device

Autor:	Darius G. Rackus, Petra Jusková, Fumiaki Yokoyama, Petra S. Dittrich
Rok vydání:	2022
Předmět:	Fluid Flow and Transfer Processes Colloid and Surface Chemistry Biomedical Engineering General Materials Science Condensed Matter Physics
Zdroj:	Biomicrofluidics, 16 (4)
ISSN:	1932-1058
DOI:	10.1063/5.0091704
Popis:	Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency. Published under an exclusive license by AIP Publishing. Biomicrofluidics, 16 (4) ISSN:1932-1058
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7fb39e5482104f6c4b00352b1bd422c https://doi.org/10.1063/5.0091704 Zobrazit plný text záznamu