Prevalence of resistance mutations associated with integrase inhibitors in therapy-naive HIV-positive patients in Hungary
| Autor: | Mária Mezei, János Szlávik, Botond Lakatos, Ágnes Pocskay, Janos Minarovits, Eva Ay |
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| Rok vydání: | 2021 |
| Předmět: |
Genotype
medicine.medical_treatment Population Integrase inhibitor HIV Infections Drug resistance Nucleoside Reverse Transcriptase Inhibitor 03 medical and health sciences 0302 clinical medicine Drug Resistance Viral Prevalence Medicine Humans 030212 general & internal medicine 03.02. Klinikai orvostan HIV Integrase Inhibitors education 0303 health sciences education.field_of_study Hungary Protease General Immunology and Microbiology biology 030306 microbiology business.industry 01.06. Biológiai tudományok General Medicine Virology Reverse transcriptase Integrase Mutation biology.protein HIV-1 business HIV drug resistance |
| Zdroj: | Acta microbiologica et immunologica Hungarica. 68(2) |
| ISSN: | 1588-2640 |
| Popis: | Widespread introduction of HIV integrase inhibitors into clinical care may result in appearance of drug resistance mutations affecting treatment outcome. The aim of our study was to monitor the resistance patterns of integrase inhibitors beside protease and reverse transcriptase inhibitors in newly diagnosed therapy-naive HIV-positive patients in Hungary between 2017 and 2019.Genotype-based resistance testing of HIV integrase, protease and reverse transcriptase was performed by amplification and Sanger population sequencing from plasma samples. Drug resistance mutations were identified by the algorithm of Stanford HIV Drug Resistance Database.Potentially transmitted, non-polymorphic integrase major mutation was detected in 1 out of 249 samples, while accessory mutations were observed in further 31 patients (12.4%). The overall prevalence of transmitted drug resistance (TDR) mutations related to protease and reverse transcriptase inhibitors was 5.8% (10/173) between the end of 2017 and 2019. Nucleoside reverse transcriptase inhibitor associated resistance mutations were the most frequent indicators of TDR (6/173; 3.5%), followed by resistance mutations associated with protease (3/173; 1.7%) and non-nucleoside reverse transcriptase inhibitors (2/173, 1.2%).The first detection of integrase major mutation and the changing patterns of other resistance mutations in Hungarian untreated HIV-positive population indicate the necessity of continuous molecular surveillance of Hungarian HIV epidemic. |
| Databáze: | OpenAIRE |
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