A Comparative Evaluation of Six Principal IgY Antibody Extraction Methods
| Autor: | Hao Ren, Xiaoying Zhang, Wenjing Yang, Diraviyam Thirumalai, Rüdiger Schade |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromatography 010401 analytical chemistry Extraction (chemistry) Caprylic acid Immunoglobulins Phenol extraction General Medicine Polyethylene glycol Toxicology 01 natural sciences General Biochemistry Genetics and Molecular Biology 0104 chemical sciences Dilution Carrageenan 03 medical and health sciences Medical Laboratory Technology chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry PEG ratio Animals Phenol Chickens |
| Zdroj: | Alternatives to Laboratory Animals. 44:11-20 |
| ISSN: | 2632-3559 0261-1929 |
| Popis: | Egg yolk has been considered a promising source of antibodies. Our study was designed to compare six principal IgY extraction methods (water dilution, polyethylene glycol [PEG] precipitation, caprylic acid extraction, chloroform extraction, phenol extraction, and carrageenan extraction), and to assess their relative extraction efficiencies and the purity of the resulting antibodies. The results showed that the organic solvents (chloroform or phenol) minimised the lipid ratio in the egg yolk. The water dilution, PEG precipitation and caprylic acid extraction methods resulted in high yields, and antibodies purified with PEG and carrageenan exhibited high purity. Our results indicate that phenol extraction would be more suitable for preparing high concentrations of IgY for non-therapeutic usage, while the water dilution and carrageenan extraction methods would be more appropriate for use in the preparation of IgY for oral administration. |
| Databáze: | OpenAIRE |
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