mRNA decapping factor Dcp1a is essential for embryonic growth in mice
| Autor: | Megumi Ibayashi, Satoshi Tsukamoto, Ryutaro Aizawa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Heart Defects Congenital Male Transgene Biophysics Mice Transgenic Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Endoribonucleases Animals Molecular Biology Gene Regulation of gene expression Messenger RNA Embryogenesis Gene Expression Regulation Developmental Embryo Heart Cell Biology Phenotype Embryonic stem cell Mice Mutant Strains Cell biology Mice Inbred C57BL 030104 developmental biology 030220 oncology & carcinogenesis Trans-Activators Female CRISPR-Cas Systems |
| Zdroj: | Biochemical and biophysical research communications. 555 |
| ISSN: | 1090-2104 |
| Popis: | mRNA decapping is a critical step in posttranscriptional regulation of gene expression in eukaryotes. Although Dcp1a is a well characterized and widely conserved mRNA decapping factor, little is known about its physiological function. To extend our understanding of Dcp1a function in vivo, we employed a transgenic rescue strategy to produce Dcp1a-deficient mice using the CRISPR/Cas9 system. This approach arrowed us to generate heterozygous Dcp1a mice and define the phenotype of Dcp1a-deficient embryos. We found that expression of Dcp1a protein, which is detectable in most mouse tissues, was developmentally regulated through embryonic growth, and that depletion of the Dcp1a gene resulted in embryonic lethality around embryonic day 10.5 (E10.5) concomitant with massive growth retardation and cardiac developmental defects. Moreover, the embryonic lethality was fully rescued by transgenic expression of exogenous human Dcp1a. Together, our results suggest that Dcp1a is required for embryonic growth. |
| Databáze: | OpenAIRE |
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