Extremely low frequency magnetic field exposure affects DnaK and GroEL expression in E. coli cells with impaired heat shock response
| Autor: | Pamela Marcantonio, Gianfranco Giorgi, Ferdinando Bersani, Del Re B |
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| Přispěvatelé: | Del Re B., Marcantonio P., Bersani F., Giorgi G. |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
HEAT SHOCK PROTEIN
Physiology Extremely low frequency magnetic field Biophysics Biology medicine.disease_cause SINUSOIDAL MAGNETIC FIELD Magnetics Heat shock protein medicine HSP70 Heat-Shock Proteins Heat shock Escherichia coli GroEL Protein Escherichia coli Proteins Temperature Wild type EXTREMELY LOW FREQUENCY MAGNETIC FIELD Chaperonin 60 Gene Expression Regulation Bacterial General Medicine respiratory system GroEL Molecular biology Shock (circulatory) TRANSPOSITION bacteria ESCHERICHIA COLI medicine.symptom Heat-Shock Response |
| Popis: | In our earlier experiments, we found that extremely low frequency magnetic fields (ELF-MF) affect heat shock protein (HSP) expression in wild type Escherichia coli cells. In the present work we investigate the ability of ELF-MF exposure to trigger an increase of DnaK and GroEL protein levels also in E. coli cells not exhibiting the classic heat shock response (HSR) when subjected to a 42 degrees C heat stress. We find that these cells, although lacking a HSR to heat shock treatment, show an enhancement of DnaK and GroEL protein levels after 30 or 90 min sinusoidal ELF-MF exposure (50 Hz, 1 mT). This result suggests that the HSP induction pathway triggered by ELF-MF exposure could be different from that elicited by heat shock treatment. |
| Databáze: | OpenAIRE |
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