In vitro antiproliferative and antimicrobial activity of the essential oil from the flowers and leaves of Helichrysum italicum (Roth) G. Don growing in Central Dalmatia (Croatia)
| Autor: | Marinko Petrovic, Marija Dinarina-Sablic, Andrea Vulinovic, Ivana Ratkaj, Mladenka Malenica Staver, Dalibor Broznić, Ivana Gobin |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Helichrysum italicum (Roth) G. Don essential oil cell cycle apoptosis antimicrobial activity 01 natural sciences Biochemistry Helichrysum italicum Analytical Chemistry law.invention HeLa 03 medical and health sciences law Viability assay Don Essential oil biology Traditional medicine 010405 organic chemistry Chemistry BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences Organic Chemistry Cell cycle Helichrysum italicum (Roth) G Antimicrobial biology.organism_classification 0104 chemical sciences 030104 developmental biology Apoptosis Cell culture BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti |
| Zdroj: | Journal of Essential Oil Bearing Plants Volume 21 Issue 1 |
| ISSN: | 0972-060X |
| Popis: | In the present paper, chemical composition, in vitro antimicrobial and antiproliferative activity of the essential oil from the flowers and leaves of Helichrysum italicum (Roth) G. Don (Central Dalmatia, Croatia) as a potential replacement for standard antibiotics and chemotherapeutic agents was analyzed. Essential oil was isolated by steam distillation and analyzed by GC/MS. Antimicrobial activity was carried out by agar-well diffusion and microdilution assays with Gram (+), Gram (-) bacteria and one yeast. Antiproliferative effect, apoptosis induction and cell death on cancer cell lines: HeLa, MCF-7, SW620, CFPAC-1 and MIA PaCa-2 were analyzed by cell viability and Annexin-V assay as well as by flow cytometric analysis. In essential oil, α-pinene, γ-curcumene and neryl acetate was found as major compounds. The antimicrobial assays, showed that essential oil had weak to moderate antimicrobial potential with S. aureus and S. epidermidis as the most sensitive bacterial strains. Essential oil treatment possessed moderate antiproliferative impact on MCF-7 and HeLa cell lines, while analyzing cell cycle treatment had no significant effect on tested cells except on MIA PaCa-2 with the highest cells increase in sub G1 phase cell cycle. However, treatment caused significantly induction of apoptosis in MCF-7 and HeLa cells, but not in MIA PaCa-2 cells. In this cell line, multiple cell death mechanism existed with involving apoptosis, senescence or necrosis. Results of the study provide a promising basis for the evaluation of the potential use of essential oil from H. italicum (Roth) G. Don as a source of alternative nature antibiotics or anticancer agents for the prevention or treatment of different diseases. |
| Databáze: | OpenAIRE |
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