MicroRNA‐379 mediates pigmentation, migration and proliferation of melanocytes by targeting the insulin‐like growth factor 1 receptor

Autor:	Bo Liu, Qi Shuhui, Junzhen Zhang, Qiong Jia, Shixiong Hu, Kaiyuan Ji, Shanshan Yang, Wanyun Yang, Changsheng Dong, Xuexian Liu, Ruiwen Fan
Rok vydání:	2020
Předmět:	0301 basic medicine Dermatology Melanocyte Polymerase Chain Reaction Biochemistry Melanocyte migration Receptor IGF Type 1 Melanin Mice 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Animals 3' Untranslated Regions Molecular Biology Protein kinase B Cell Proliferation Insulin-like growth factor 1 receptor Melanins Microphthalmia-Associated Transcription Factor Activating Transcription Factor 2 Pigmentation Chemistry Microphthalmia-associated transcription factor Receptor Insulin Cell biology MicroRNAs Phenotype 030104 developmental biology medicine.anatomical_structure Melanocytes Dendrite extension Melanocyte proliferation Camelids New World Protein Binding
Zdroj:	Experimental Dermatology. 29:467-476
ISSN:	1600-0625 0906-6705
DOI:	10.1111/exd.14095
Popis:	Melanogenesis, migration and proliferation of melanocytes are important factors that determine the hair colours of mammals. MicroRNAs (miRNAs) have been shown to be closely related to these processes. In melanocytes of alpacas, insulin-like growth factor 1 (IGF1) has been shown to improve melanogenesis through the cyclic AMP (cAMP) pathway. miR-379 was predicted to target insulin-like growth factor (IGF) receptor 1 (IGF1R), which binds to IGF1. Therefore, we hypothesized that miR-379 could mediate melanogenesis, migration and proliferation of melanocytes. Here, we report that miR-379 was highly expressed in alpaca melanocytes. Subsequent overexpression of miR-379 in alpaca melanocytes led to the generation of the phenotype of melanogenesis, proliferation and migration. In addition, the expression of genes related to these phenotypes in melanocytes was detected. Our results showed that miR-379 targets IGF1R in melanocytes. The overexpression of miR-379 stimulated dendrite extension or elongation and limited the perinuclear distribution of melanin, but inhibited melanogenesis via cAMP response element (CRE)-binding protein (CREB)/microphthalmia-associated transcription factor (MITF) pathway. miR-379 attenuated melanocyte migration by downregulating the focal adhesion kinase (FAK) and enhanced melanocyte proliferation by upregulating protein kinase B (AKT). These observations suggest the involvement of miR-379 in the physiological regulation of melanocytes, mediated by targeting IGF1R on insulin receptor (IR) compensation and subsequent crosstalk.
Databáze:	OpenAIRE
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