Characterization of Fibroblasts with Son of Sevenless-1 Mutation
| Autor: | Thomas C. Hart, J. Kather, Eun Jin Lee, S.I. Jang, Debora Pallos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
0301 basic medicine
Adult G2 Phase Gingiva Matrix (biology) Biology Article Flow cytometry S Phase Extracellular matrix 03 medical and health sciences 0302 clinical medicine medicine Cell Adhesion Humans Fibroblast Frameshift Mutation General Dentistry Cell Proliferation Fibromatosis Gingival medicine.diagnostic_test Cell growth Fibromatosis 030206 dentistry Fibroblasts medicine.disease Molecular biology Hereditary gingival fibromatosis Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Case-Control Studies SOS1 Collagen SOS1 Protein |
| Popis: | Although non-syndromic hereditary gingival fibromatosis (HGF) is genetically heterogeneous, etiologic mutations have been identified only in the Son of Sevenless-1 gene ( SOS1). To test evidence of increased cell proliferation, we studied histological, morphological, and proliferation characteristics in monolayer and three-dimensional cultures of fibroblasts with the SOS1 g.126,142–126,143insC mutation. Histological assessment of HGF gingiva indicated increased numbers of fibroblasts (30%) and increased collagen (10%). Cell proliferation studies demonstrated increased growth rates and 5-bromo-2-deoxyuridine incorporation for HGF fibroblasts. Flow cytometry showed greater proportions of HGF fibroblasts in the G2/M phase. Attachment of HGF fibroblasts to different extracellular matrix surfaces demonstrated increased formation of protrusions with lamellipodia. HGF fibroblasts in three-dimensional culture showed greater cell proliferation, higher cell density, and alteration of surrounding collagen matrix. These findings revealed that increased fibroblast numbers and collagen matrix changes are associated with mutation of the SOS1 gene in vitro and in vivo. |
| Databáze: | OpenAIRE |
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