High discrimination of Mycobacterium bovis isolates in Brazilian herds by spoligotyping
| Autor: | Marcos Bryan Heinemann, Beatriz Romero Martínez, Vivianne Cambuí Figueiredo Rocha, Lucía de Juan Ferré, Pedro Moacyr Pinto Coelho Mota, Cássia Yumi Ikuta, José Henrique Hildebrand e Grisi Filho, Marina de Azevedo Issa, José Soares Ferreira Neto, Lucas José Domínguez Rodríguez, A. F. Souza-Filho |
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| Rok vydání: | 2020 |
| Předmět: |
Tuberculosis
040301 veterinary sciences 030231 tropical medicine 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Food Animals medicine Animals Cluster Analysis Typing Genotyping Spatial Analysis Mycobacterium bovis biology Molecular epidemiology Zoonosis 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Virology Molecular Typing TUBERCULOSE DNA profiling Infectious disease (medical specialty) Epidemiological Monitoring Cattle Animal Science and Zoology Tuberculosis Bovine Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0167-5877 |
| Popis: | Bovine tuberculosis is an infectious disease caused by Mycobacterium bovis (M. bovis), that leads to economic losses in infected herds and it is also considered an important zoonosis. The molecular typing methods of M. bovis isolates are fundamental for the bovine tuberculosis surveillance system, and spoligotyping is the standard genotyping technique for this species. Thus, the aim of the present study is to analyze the spatial and cluster distribution of M. bovis strains from several regions of Brazil through molecular typing. Spoligotyping technique was applied on 422 isolates identified as M. bovis, and Ripley's K function was used to perform the spatial and cluster analysis of each identified profile. Forty-three (43) different profiles were identified and spoligotype SB0121 was the most frequent and showed a uniform pattern in the spatial distribution while spoligotypes SB0295, SB1380 and SB1050 formed clusters. In addition, three novel spoligotype profiles (SB2361, SB2362, SB2364) were identified in different herds. In this perspective, it is believed that molecular identification and typing can significantly improve the performance of surveillance systems for bovine tuberculosis in Brazil. |
| Databáze: | OpenAIRE |
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