Multiparameter Affinity Microchip for Early Sepsis Diagnosis Based on CD64 and CD69 Expression and Cell Capture
| Autor: | Dimitri Pappas, Yun Zhou, Amanda Johnson, Ye Zhang, Amanda Venable, Veronica J. Lyons, Wenjie Li, John A. Griswold |
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| Rok vydání: | 2018 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Surface Properties Cell chemical and pharmacologic phenomena Cell Separation Analytical Chemistry Flow cytometry Sepsis 03 medical and health sciences 0302 clinical medicine Antigen Antigens CD medicine Humans Lectins C-Type 030212 general & internal medicine IL-2 receptor Receptor CD64 medicine.diagnostic_test biology Chemistry Receptors IgG hemic and immune systems 030208 emergency & critical care medicine Microfluidic Analytical Techniques medicine.disease Flow Cytometry Molecular biology medicine.anatomical_structure Early Diagnosis biology.protein Antibody |
| Zdroj: | Analytical chemistry. 90(12) |
| ISSN: | 1520-6882 |
| Popis: | Sepsis is a leading cause of death worldwide. In this work, a multiparameter affinity microchip was developed for faster sepsis diagnosis, which can reduce the mortality caused by late validation. The separation device captured cells expressing CD25, CD64, and CD69 into discrete antibody regions. The performance of multiparameter cell separation microchips was compared with flow cytometry analysis and validated with samples of septic patients ( n = 15) and healthy volunteers ( n = 10). The total analysis time was 2 h. Results showed that total on-chip cell counts for both CD64 and CD69 regions were linear with antigen expression levels. The difference between cell capture for septic and healthy samples was statistically significant (CD64: p = 0.0033; CD69: p = 0.0221, 95% confidence interval), indicating that sepsis is distinguishable based on microfluidic cell capture. For on-chip detection of CD64+ and CD69+ leukocytes, the AUC was 0.95 and 0.78, respectively. The combination of CD64 and CD69 for sepsis diagnosis had the AUC of 0.98, indicating the improved and excellent diagnostic performance of multiple parameters. |
| Databáze: | OpenAIRE |
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