High-throughput Sequencing Analysis of the Rhizosphere Arbuscular Mycorrhizal Fungi (AMF) Community Composition Associated with Ferula Sinkiangensis
| Autor: | Li Zhuang, Yaling He, Zhongke Wang, Yunfeng Luo, Xinhua Lv, Guifang Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Microbiology (medical) Soil test lcsh:QR1-502 01 natural sciences Microbiology lcsh:Microbiology Soil Nutrient Mycology Mycorrhizae Community composition Organic matter DNA Fungal Glomeromycota Glomus Soil Microbiology chemistry.chemical_classification Rhizosphere Diversity Plants Medicinal biology fungi Soil physiochemical High-Throughput Nucleotide Sequencing 04 agricultural and veterinary sciences Biodiversity Sequence Analysis DNA Ferula sinkiangensis Illumina MiSeq biology.organism_classification Arbuscular mycorrhizal fungi (AMF) Ferula Arbuscular mycorrhiza Horticulture chemistry Soil water 040103 agronomy & agriculture 0401 agriculture forestry and fisheries 010606 plant biology & botany Research Article Mycobiome |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 20, Iss 1, Pp 1-14 (2020) |
| Popis: | Background Ferula sinkiangensis is an increasingly endangered medicinal plant. Arbuscular mycorrhiza fungi (AMF) are symbiotic microorganisms that live in the soil wherein they enhance nutrient uptake, stress resistance, and pathogen defense in host plants. While such AMF have the potential to contribute to the cultivation of Ferula sinkiangensis, the composition of AMF communities associated with Ferula sinkiangensis and the relationship between these fungi and other pertinent abiotic factors still remains to be clarified. Results Herein, we collected rhizosphere and surrounding soil samples at a range of depths (0–20, 20–40, and 40–60 cm) and a range of slope positions (bottom, middle, top). These samples were then subjected to analyses of soil physicochemical properties and high-throughput sequencing (Illumina MiSeq). We determined that Glomus and Diversispora species were highly enriched in all samples. We further found that AMF diversity and richness varied significantly as a function of slope position, with this variation primarily being tied to differences in relative Glomus and Diversispora abundance. In contrast, no significant relationship was observed between soil depth and overall AMF composition, although some AMF species were found to be sensitive to soil depth. Many factors significantly affected AMF community composition, including organic matter content, total nitrogen, total potassium, ammonium nitrogen, nitrate nitrogen, available potassium, total dissolvable salt levels, pH, soil water content, and slope position. We further determined that Shannon diversity index values in these communities were positively correlated with total phosphorus, nitrate-nitrogen levels, and pH values (P P Conclusion In summary, our data revealed that Glomus and Diversispora are key AMF genera found within Ferula sinkiangensis rhizosphere soil. These fungi are closely associated with specific environmental and soil physicochemical properties, and these soil sample properties also differed significantly as a function of slope position (P Ferula sinkiangensis, offering a theoretical basis for further studies of their development. |
| Databáze: | OpenAIRE |
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