Susceptibilities of Geographic Populations of Helicoverpa zea (Lepidoptera: Noctuidae) in Mexico to Bt ∂-Endotoxins Cry1Ac and Cry2Ab: An 18-Yr Study
| Autor: | Gonzalo Silva-Aguayo, José L. Martínez-Carrillo, Sotero Aguilar-Medel, J. Concepción Rodríguez |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Moths 01 natural sciences Lepidoptera genitalia Insecticide Resistance 03 medical and health sciences Hemolysin Proteins Bacterial Proteins Bacillus thuringiensis Animals Mexico Ecology biology Bacillus thuringiensis Toxins fungi General Medicine biology.organism_classification Monitoring program Endotoxins 010602 entomology 030104 developmental biology Bt cotton Cry1Ac Insect Science Noctuidae Instar Helicoverpa zea |
| Zdroj: | Journal of economic entomology. 110(5) |
| ISSN: | 1938-291X |
| Popis: | An insect resistance monitoring program was developed for Mexico to accommodate the commercial introduction and stewardship of Bt cotton. Between 1998 and 2015, field-collected geographic populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) were evaluated against Cry1Ac and Cry2Ab proteins of Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) to establish baseline susceptibility data before the commercial use of Bollgard (Cry1Ac) and Bollgard II (Cry1Ac and Cry2Ab) cotton. An annual monitoring program was subsequently established in which a single diagnostic concentration of each Bt protein was used in a diet overlay bioassay. The diagnostic concentration represented the concentration where larvae, evaluated in baseline studies, were reduced in weight by ≥97% relative to untreated controls or failed to molt to third instar after 5 d. In the monitoring study, populations were tested against Cry1Ac from 1998 through 2015, and against Cry2Ab from 2002 through 2004 and again from 2007 through 2015. None of the Cry1Ac-exposed larvae tested during the 18-yr period reached the third larval instar after an exposure of 5 d, and weight reduction relative to untreated control larvae was uniform at about 98-99%. For the 12 yr of Cry2Ab monitoring, no larvae reached third instar, and weight reduction was uniform at >97% relative to controls. These results indicate that H. zea susceptibility to Cry1Ac and Cry2Ab has not changed during the period Bollgard and Bollgard II have been cultivated in Mexico. |
| Databáze: | OpenAIRE |
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