Glucose-Induced Time-Dependent Potentiation of Insulin Release, but not Islet Blood Perfusion, in Anesthetized Rats
| Autor: | Örjan Källskog, Leif Jansson, Birgitta Bodin |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty medicine.medical_treatment Thiobutabarbital Islets of Langerhans In vivo Internal medicine medicine Animals Insulin Rats Wistar Saline geography geography.geographical_feature_category business.industry Long-term potentiation General Medicine Blood flow Islet Rats Glucose Endocrinology business Perfusion medicine.drug |
| Zdroj: | Upsala Journal of Medical Sciences. 113:47-56 |
| ISSN: | 2000-1967 0300-9734 |
| DOI: | 10.3109/2000-1967-213 |
| Popis: | BACKGROUND: Repeated administration of glucose in vivo leads to a time-dependent potentiation of insulin release. Glucose is also known to stimulate pancreatic islet blood flow, but whether this is associated with a time-dependent potentiation is unknown. We therefore repeatedly administered glucose to anesthetized rats and evaluated effects on insulin release and islet blood flow. METHODS: Male Wistar-Furth rats, anesthetized with thiobutabarbital, were injected intravenously with 1 ml of saline or glucose at times 0, 30 and 60 min. The combinations used were saline + saline + saline (SSS), glucose + saline + saline (GSS), saline + saline + glucose (SSG) and glucose + glucose + glucose (GGG). Regional organ blood flow values were measured 3 min after the final injection with a microsphere technique, and at this time also serum insulin concentrations were determined with ELISA. RESULTS: Serum insulin concentrations as well as total pancreatic, pancreatic islet and duodenal blood flow were higher in SSG and GGG-treated rats when compared to those given SSS and GSS. However, only insulin concentrations, not blood flow values, were higher in GGG rats when compared to SSG animals. CONCLUSIONS: Glucose-induced time-dependent potentiation of insulin release occurs in vivo in thiobutabarbital-anesthetized rats, but is not associated with a further increase in islet blood flow. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|