CD4-independent, productive human immunodeficiency virus type 1 infection of hepatoma cell lines in vitro
| Autor: | Xi Ling Li, M Mirabile, David D. Ho, Yunzhen Cao, T. Moudgil, D Zucker-Franklin, Alvin E. Friedman-Kien, Y X Huang |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Carcinoma
Hepatocellular medicine.drug_class Immunology In situ hybridization Biology Immunofluorescence Monoclonal antibody Microbiology Virus Cell Line Proviruses Antigen Virology Tumor Cells Cultured medicine Humans Virulence medicine.diagnostic_test Liver Neoplasms Nucleic Acid Hybridization digestive system diseases In vitro Microscopy Electron Cell culture Insect Science CD4 Antigens DNA Viral HIV-1 Immunohistochemistry Research Article |
| Zdroj: | Journal of Virology. 64:2553-2559 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.64.6.2553-2559.1990 |
| Popis: | Five hepatoma cell lines, including CZHC/8571, PLC/PRF/5, Hep3B, HepG2, and HUH7, were inoculated with three diverse isolates of human immunodeficiency virus type 1 (HIV-1). Productive infection was noted in all hepatoma cell lines, and expression of viral p24 antigen lasted for over 3 months, but its level decreased in proportion to the number of viable cells. HIV-1 antigens were also found in the cells by immunohistochemical staining and radioimmunoprecipitation assay, as were viral RNA by in situ hybridization and HIV-1-like particles by electron microscopy. Virus yield assays were also positive on supernatant fluids collected from hepatoma cultures inoculated with HIV-1. Despite their susceptibility to infection, all five hepatoma cell lines were negative for CD4 by immunofluorescence and for CD4 mRNA by slot-blot hybridization. In addition, HIV-1 infection of hepatoma cell lines was not blocked by anti-CD4 monoclonal antibody or soluble CD4. Together, these findings clearly demonstrate that all five hepatoma cell lines were susceptible to productive infection by HIV-1 in vitro via a CD4-independent mechanism. |
| Databáze: | OpenAIRE |
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