4-Methoxylonchocarpin attenuates inflammation by inhibiting lipopolysaccharide binding to Toll-like receptor of macrophages and M1 macrophage polarization
| Autor: | Dae-Sik Jang, Su-Min Lim, Thi Kim Van Le, Hyo-Min Jang, Dong-Hyun Kim, Geum-Dan Kang |
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| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Colon Immunology Anti-Inflammatory Agents Macrophage polarization Inflammation Pharmacology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Immunology and Allergy Colitis Cells Cultured Mice Inbred ICR Toll-like receptor Interleukin-6 business.industry Macrophages NF-kappa B Cell Differentiation Flavones M2 Macrophage medicine.disease Lipopolysaccharide binding Toll-Like Receptor 4 030104 developmental biology Trinitrobenzenesulfonic Acid 030220 oncology & carcinogenesis Abrus TLR4 Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | International Immunopharmacology. 45:90-97 |
| ISSN: | 1567-5769 |
| Popis: | The roots of Abrus precatorius (AP, Fabaceae) have traditionally been used in Vietnam and China for the treatment of inflammatory diseases such as stomatitis, asthma, bronchitis, and hepatitis. Therefore, in this study, we isolated 4-methoxylonchocarpin (ML), an anti-inflammatory compound present in AP, and studied its anti-inflammatory effects in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. In lipopolysaccharide (LPS)-stimulated macrophages, ML was found to inhibit nuclear factor (NF)-κB activation and tumor necrosis factor (TNF) and interleukin (IL)-6 expression by inhibiting LPS binding to Toll-like receptor 4 (TLR4) in vitro. Oral administration of ML in mice with TNBS-induced colitis suppressed colon shortening and colonic myeloperoxidase activity. ML treatment significantly inhibited the activation of nuclear factor (NF)-κB and phosphorylation of transforming growth factor β-activated kinase 1 in the colon. Treatment with ML also inhibited TNBS-induced expression of IL-1β, IL-17A, and TNF. While ML reduced the TNBS-induced expression of M1 macrophage markers such as arginase-2 and TNF, it was found to increase the expression of M2 macrophage markers such as arginase-1 and IL-10. In conclusion, oral administration of ML attenuated colitis in mice by inhibiting the binding of LPS to TLR4 on immune cells and increasing the polarization of M1 macrophages to M2 macrophages. |
| Databáze: | OpenAIRE |
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