Modulation of the Na(+)-H(+) antiport activity by adrenaline on erythrocytes from normal and obese individuals
| Autor: | Dimitris Bourikas, Maria Bougoulia, Zacharoula Zolota, George Koliakos, Martha Kaloyianni |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Erythrocytes Sodium-Hydrogen Exchangers Time Factors Adrenergic receptor Epinephrine Intracellular pH Stimulation Propranolol Biochemistry Body Mass Index Amiloride chemistry.chemical_compound Endocrinology Internal medicine Phorbol Esters medicine Humans Calphostin Obesity Molecular Biology Protein kinase C Chemistry Staurosporine Phorbol medicine.drug |
| Zdroj: | Molecular and cellular endocrinology. 205(1-2) |
| ISSN: | 0303-7207 |
| Popis: | The effect of adrenaline on normal and obese human Na + –H + antiport (NHE 1) erythrocyte activity has been studied. Adrenaline increased both intracellular pH (pHi) and Na + influx in erythrocyte suspensions. This effect of adrenaline was inhibited by amiloride or EIPA, indicating that adrenaline stimulated NHE 1. Phorbol myristicate ester (PMA), a protein kinase C (PKC) stimulator, increased the activity of NHE 1 whereas calphostin C, a PKC inhibitor, partially inhibited NHE 1 activation induced by adrenaline. The effect of adrenaline to NHE 1 was counteracted by prazocin and by propranolol as well indicating the involvement of both alpha and beta 2 adrenergic receptors. The effect of adrenaline on erythrocyte NHE 1 activity was significantly more profound in obese compared to normal subjects. These data indicate that adrenaline induces an increase of pHi and Na + uptake of human erythrocytes through stimulation of NHE 1 activity. The significantly more profound stimulation of NHE 1 activity by adrenaline in obese as compared to normal subjects is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect