Tuning reversible cell adhesion to methacrylate‐based thermoresponsive polymers: Effects of composition on substrate hydrophobicity and cellular responses
| Autor: | Lauren S. Sefcik, Katarina M. DiLillo, James K. Ferri, Christopher R. Anderson, Erik Wischerhoff, Filippo Gambinossi, André Laschewsky |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Polymers Cell Biomedical Engineering 02 engineering and technology 010402 general chemistry Cell morphology 01 natural sciences Cell Line Biomaterials Mice Polymer chemistry Cell Adhesion medicine Animals Thermoresponsive polymers in chromatography Cell adhesion Cell Shape Focal Adhesions Temperature Metals and Alloys Substrate (chemistry) Blood Proteins Adhesion Fibroblasts 021001 nanoscience & nanotechnology 0104 chemical sciences Kinetics medicine.anatomical_structure Gene Expression Regulation Cell culture Ceramics and Composites Biophysics Methacrylates Adsorption 0210 nano-technology Hydrophobic and Hydrophilic Interactions Protein adsorption |
| Zdroj: | Journal of Biomedical Materials Research Part A. 105:2416-2428 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.36100 |
| Popis: | Thermoresponsive polymer (TRP) cell culture substrates are widely utilized for nonenzymatic, temperature-triggered release of adherent cells. Increasingly, multicomponent TRPs are being developed to facilitate refined control of cell adhesion and detachment, which requires an understanding of the relationships between composition-dependent substrate physicochemical properties and cellular responses. Here, we utilize a homologous series of poly(MEO2 MAx -co-OEGMAy ) brushes with variable copolymer ratio (x/y) to explore the effects of substrate hydrophobicity on L-929 fibroblast adhesion, morphology, and temperature-triggered cell detachment. Substrate hydrophobicity is reported in terms of the equilibrium spreading coefficient (S), and variations in copolymer ratio reveal differential hydrophobicity that is correlated to serum protein adsorption and initial cell attachment at 37°C. Furthermore, quantitative metrics of cell morphology show that cell spreading is enhanced on more hydrophobic surfaces with increased (x/y) ratio, which is further supported by gene expression analysis of biomarkers of cell spreading (e.g., RhoA, Dusp2). Temperature-dependent cell detachment is limited for pure poly(MEO2 MA); however, rapid cell rounding and detachment ( |
| Databáze: | OpenAIRE |
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