A Proteomics Study on the Mechanism of Nutmeg-Induced Hepatotoxicity
| Autor: | Xiaoyu Zhang, Zhipeng Cao, Lina Gao, Wei Xia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Hallucinogen
Male hepatotoxicity CYP450s Pharmaceutical Science Pharmacology Biology Proteomics medicine.disease_cause 01 natural sciences Article Analytical Chemistry Myristica Lipid peroxidation lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound Mice proteomics Cytochrome P-450 Enzyme System lcsh:Organic chemistry Drug Discovery medicine Animals nutmeg oxidative stress Physical and Theoretical Chemistry 030304 developmental biology chemistry.chemical_classification Liver injury 0303 health sciences Glutathione Peroxidase Glutathione peroxidase 010401 analytical chemistry Organic Chemistry Nutmeg lipid peroxidation medicine.disease Malondialdehyde biology.organism_classification 0104 chemical sciences chemistry Chemistry (miscellaneous) Seeds Molecular Medicine Chemical and Drug Induced Liver Injury Oxidative stress |
| Zdroj: | Molecules, Vol 26, Iss 1748, p 1748 (2021) Molecules Volume 26 Issue 6 |
| ISSN: | 1420-3049 |
| Popis: | Nutmeg is a traditional spice and medicinal plant with a variety of pharmacological activities. However, nutmeg abuse due to its hallucinogenic characteristics and poisoning cases are frequently reported. Our previous metabolomics study proved the hepatotoxicity of nutmeg and demonstrated that high-dose nutmeg can affect the synthesis and secretion of bile acids and cause oxidative stress. In order to further investigate the hepatotoxicity of nutmeg, normal saline, 1 g/kg, 4 g/kg nutmeg were administrated to male Kunming mice by intragastrical gavage for 7 days. Histopathological investigation of liver tissue, proteomics and biochemical analysis were employed to explore the mechanism of liver damage caused by nutmeg. The results showed that a high-dose (4 g/kg) of nutmeg can cause significant increased level of CYP450s and depletion of antioxidants, resulting in obvious oxidative stress damage and lipid metabolism disorders but this change was not observed in low-dose group (1 g/kg). In addition, the increased level of malondialdehyde and decreased level of glutathione peroxidase were found after nutmeg exposure. Therefore, the present study reasonably speculates that nutmeg exposure may lead to liver injury through oxidative stress and the degree of this damage is related to the exposure dose. |
| Databáze: | OpenAIRE |
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