Differentiation of Acanthamoeba strains from infected corneas and the environment by using restriction endonuclease digestion of whole-cell DNA
| Autor: | S. Kilvington, John R. Beeching, D G White |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Microbiology (medical)
Contact Lenses EcoRI Acanthamoeba HindIII Microbiology Species Specificity medicine Animals Humans Soil Microbiology BglII biology DNA Protozoan biology.organism_classification medicine.disease Contact lens Restriction enzyme Acanthamoeba Keratitis Acanthamoeba keratitis Evaluation Studies as Topic biology.protein Parasitology Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | Journal of Clinical Microbiology. 29:310-314 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.29.2.310-314.1991 |
| Popis: | Restriction endonuclease digestion of Acanthamoeba whole-cell DNA was used to study the relationship between 33 morphologically identical strains from keratitis cases (30 strains), contact lens storage containers (2 strains), and soil (1 strain). Samples digested with BglII, EcoRI, or HindIII and separated by agarose gel electrophoresis contained detectable mitochondrial DNA restriction fragment length polymorphisms (RFLPs). By comparing RFLPs, the strains could be assigned to seven multiple-strain and three single-strain groups. The largest of these contained nine strains, eight of which were isolated in keratitis cases in various locations worldwide and may indicate a group particularly associated with keratitis. Restriction endonuclease analysis of whole-cell DNA is proposed as a valuable technique for detecting mitochondrial DNA RFLPs in the differentiation of morphologically identical Acanthamoeba strains and may therefore be useful in resolving the complex taxonomy of the genus, which has hitherto been founded on subjective morphological criteria. |
| Databáze: | OpenAIRE |
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