DISCRIMINATION OF HLA-B5 CROSSREACTIVE GROUP ANTIGENS BY HUMAN ALLOSPECIFIC CTL CLONES
| Autor: | Masaki Hiraiwa, Kyoichi Kano, Kazuhiko Matsumoto, Junji Yamamoto, Masafumi Takiguchi |
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| Rok vydání: | 1990 |
| Předmět: |
Transplantation
Alpha (ethology) T lymphocyte Human leukocyte antigen Cross Reactions In Vitro Techniques Biology Virology Epitope In vitro Clone Cells stomatognathic diseases CTL Antigen HLA-B Antigens HLA-B51 Antigen Humans HLA-B35 Antigen HLA-B52 Antigen Clone (B-cell biology) T-Lymphocytes Cytotoxic |
| Zdroj: | Transplantation. 49:1164-1167 |
| ISSN: | 0041-1337 |
| Popis: | Human CTL clones discriminating serologically closely related HLA-Bw52, B51, and B35, which belong to HLA-B5 crossreacting group (CREG), were established from peripheral blood lymphocytes by repeated in vitro stimulations. Five HLA-Bw52-specific CTL clones from an individual with HLA-B5 CREG antigens and four CTL clones from another individual with HLA-B51 were generated. The specificity of these CTL clones was ascertained by their lysis of EBV-transformed B cells with HLA-Bw52, but not those with HLA-B51 or B35, and Bw52-transfected Hmy2CIR cells but not HLA-B51 or B35 transfectants. Conversely HLA-B51-specific clones were generated from the HLA-B5 CREG-negative individual, as well as another individual with HLA-Bw52. Their specificity was determined in a similar fashion. Since HLA-B51 differed from HLA-Bw52 only by two amino acid substitutions on the alpha helical region of the alpha 1 domain, these results demonstrated that allospecific CTLs can be produced and discriminate the epitopes formed by the subtle difference in the structure of these HLA class I molecules. Furthermore, three HLA-B35-specific CTL clones were generated from the HLA-B5 CREG-negative individual that discriminated HLA-B35 from HLA-Bw52 and B51. Taken together these results demonstrated that human CTL clones could definitively discriminate the three serologically related HLA-B5 CREG specificities. |
| Databáze: | OpenAIRE |
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