Hyperthermia enhances cytomegalovirus regulation of HIV-1 and TNF-α gene expression
| Autor: | Pope L. Moseley, Audrey M. Ainsworth, Gary K. Iwamoto |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Gene Expression Regulation
Viral Pulmonary and Respiratory Medicine Human cytomegalovirus Hyperthermia Fever Transcription Genetic Physiology Cytomegalovirus Biology Transfection Monocytes Proinflammatory cytokine Physiology (medical) Heat shock protein Gene expression Tumor Cells Cultured medicine Humans HSP70 Heat-Shock Proteins THP1 cell line Promoter Regions Genetic Acquired Immunodeficiency Syndrome Leukemia Tumor Necrosis Factor-alpha virus diseases Cell Biology medicine.disease Cytomegalovirus Infections Immunology HIV-1 Cancer research Tumor necrosis factor alpha |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 277:L1051-L1056 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1999.277.5.l1051 |
| Popis: | The immediate-early (IE) genes of human cytomegalovirus (CMV) can be expressed in monocytic cells and are known to regulate viral and cellular genes. Reactivation of human immunodeficiency virus (HIV-1) may be stimulated by a variety of factors including other viruses and inflammatory cytokines. These studies examine the role of hyperthermia and CMV in the regulation of HIV-1 and tumor necrosis factor (TNF)-α. THP-1 cells were transfected with the CMV IE genes. HIV-1 and TNF-α transcription were assessed with chloramphenicol acetyltransferase promoter constructs. Hyperthermia sufficient to stimulate production of heat shock proteins was used to stimulate the cells. Hyperthermia significantly enhances the effect of CMV IE gene products on the expression of HIV-1 and TNF-α. The increases in HIV-1 transcription appear to be in part due to increases in TNF-α. Heat shock proteins induced by hyperthermia may play an important role in the viral regulation of monocytic function by CMV. |
| Databáze: | OpenAIRE |
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