Polyphenol composition and antioxidant activity of Searsia tripartita and Limoniastrum guyonianum growing in Southeastern Algeria
| Autor: | Alain Bouchereau, Fatima Ghouini, Soumia Hadjadj, Sylvain Guyot, Aminata Ould El Hadj-Khelil, Marie-Andrée Esnault, Razika Lamini, Solenne Berardocco |
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| Přispěvatelé: | Université Kasdi Merbah Ouargla, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the University KASDI Merbah-Ouargla, Algeria, the International Program between Franceand Algeria (Tassili 11MDU822) and by the Institut for Genetics, Environment and Plant Protection (IGEPP), INRA, France, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Rok vydání: | 2020 |
| Předmět: |
DPPH
Limoniastrum guyonianum Ethyl acetate [CHIM.THER]Chemical Sciences/Medicinal Chemistry 01 natural sciences chemistry.chemical_compound Rutin Maceration (wine) Gallic acid lcsh:Science Flavonoids Multidisciplinary ABTS Traditional medicine 010405 organic chemistry 010401 analytical chemistry Polyphenols [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics Antioxidant capacity 0104 chemical sciences chemistry Phytochemical Polyphenol lcsh:Q Searsia tripartita Tannins |
| Zdroj: | Scientific African Scientific African, Elsevier, 2020, 10, pp.e00585. ⟨10.1016/j.sciaf.2020.e00585⟩ Scientific African, Vol 10, Iss, Pp e00585-(2020) Scientific African, 2020, 10, pp.e00585. ⟨10.1016/j.sciaf.2020.e00585⟩ |
| ISSN: | 2468-2276 |
| DOI: | 10.1016/j.sciaf.2020.e00585 |
| Popis: | International audience; Due to the notable medicinal value of Searsia tripartita and Limoniastrum guyonianum, it was of great interest to conduct phytochemical and antioxidant investigations. Polyphenol extracts of the dried powder of plants were prepared by maceration in 70% of methanol. The concentrated extracts were successively fractioned with distilled water, ethyl acetate and n-butanol. Obtained extracts were analysed for their phenol compositions by colorimetric processes and were evaluated for their total antiradical capacity by ABTS, DPPH and ORAC assays. Additionally, the phytochemical components were characterized in the methanolic extracts by HPLC–DAD–ESI-MS/MS in the negative ionisation mode. S. tripartita exhibited the higher quantity of phenolic compounds. The ethyl acetate extract of S. tripartita had the greatest quantities of polyphenols (55.5 ± 4.9 mg gallic acid equivalent per gram of dry residue (GAE/g DR)), flavonoids (44.2 ± 0.8 mg rutin equivalent per gram of dry residue (RE/g DR)), and tannins (12.5 ± 2.5 mg GAE/g DR). In L. guyonianum, the aqueous fraction had the highest quantity of polyphenols and tannins (10.2 ± 1.4 and 3.2 ± 1.9 mg GAE/g DR, respectively), whereas flavonoids (1.8 ± 0.2 mg RE/g DR) were higher in the ethyl acetate portion. The antioxidant capacity of the hydromethanolic extract of S. tripartita was found to be 3–16 times more effective than that of L. guyonianum using ORAC, DPPH, ABTS tests. HPLC analysis of hydromethanolic extracts provided tentative identification of four flavonoid glycosides in S. tripartita (myricetin-3-O-glucoside, myricetin-3-O-rhamnoside, quercetin-3-O-glucoside, and quercetin-3-O-rhamnoside) and five phenolic metabolites of the flavonoid class in L. guyonianum (myricetin-3-O-rhamnosylglucoside, myricetin-3-O-glucuronide, myricetin-3-O-pentoside, quercetin-3-O-glucuronide, and eriodictyol-7-O-rutinoside). Our findings revealed that these plants could be used as a potent source of health molecules. |
| Databáze: | OpenAIRE |
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