Isolation and detailed characterization of human cell lines resistant to D-threo-chloramphenicol
| Autor: | W. Sly, R. L. Siegel, A.J. Jeffreys, I. W. Craig |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Drug Resistance
Glucuronates Carbomycin Leucomycins Virginiamycin HeLa chemistry.chemical_compound medicine Inner mitochondrial membrane Methionine biology Dose-Response Relationship Drug Chloramphenicol Acetylation Cell Biology biology.organism_classification Molecular biology Clone Cells Mitochondria chemistry Biochemistry Cell culture Karyotyping Protein Biosynthesis Mutation Glucuronide Cell Division medicine.drug HeLa Cells |
| Zdroj: | Experimental cell research. 102(2) |
| ISSN: | 0014-4827 |
| Popis: | The isolation and characterization of chloramphenicol resistant derivatives of the human cell line HeLa B is described. Growth of resistant lines was unaffected in the presence of 100 μg/ml d -threo-chloramphenicol, whereas growth of the parental cells was inhibited at 12.5 μg/ml. The incorporation of [35S]methionine into mitochondrial protein of intact resistant cells continued normally in the presence of 100 μg/ml chloramphenicol (cytoplasmic protein synthesis was blocked by addition of 50 μg/ml emetine). Under these conditions the electrophoretic profile of labelled, presumptive mitochondrially-made proteins was similar to that of the parental cell line labelled in the absence of chloramphenicol. The cell lines selected in the presence of chloramphenicol also showed increased resistance to some other inhibitors of mitochondrial protein synthesis, e.g. carbomycin and mikamycin. [14C]Chloramphenicol was found to have normal access to the interior of resistant cells and it is therefore unlikely that resistance results from altered cell permeability. No modification of the drug by acetylation or glucuronide conjugation mechanisms was observed. The possibilities remain that resistance is mediated by altered permeability of the mitochondrial membrane, or from modification to a component of the mitochondrial protein synthetic system. |
| Databáze: | OpenAIRE |
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