Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L
| Autor: | Joseph Selvin, Sergio de los Santos Villalobos, Valeria Valenzuela Ruiz, Joydeep Mukherjee, Dhruba Bhattacharya |
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| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Bacillus Diketopiperazines 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents Genus RNA Ribosomal 16S Animals Molecular Biology Spongia officinalis Phylogeny Phosphatidylglycerol Bacillus (shape) biology Rugosus Strain (chemistry) Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Lipids Bacterial Typing Techniques Porifera 030104 developmental biology chemistry Bacteria |
| Zdroj: | Antonie van Leeuwenhoek. 113:1675-1687 |
| ISSN: | 1572-9699 0003-6072 |
| Popis: | A novel Gram-positive and endospore-forming bacterium assigned as strain SPB7T which is also a new source of a cyclic diketopiperazine (3S,6S)-3,6-diisobutylpiperazine-2,5-dione is described. A polyphasic (biochemical, phenotypic and genotypic) approach was used to clarify the taxonomic affiliation of this strain. The partial and complete 16S rRNA gene sequences revealed that strain SPB7T is a member of the Bacillus genus [showing high similarity (> 98.70%) with Bacillus spizizenii NRRL B-23049T, Bacillus tequilensis KCTC 13622T, Bacillus inaquosorum KCTC 13429T and Bacillus cabrialesii TE3T]. The maximum values for average nucleotide identity (ANI) and in silico DNA–DNA hybridization (GGDC, Formula 2) of strain SPB7T was obtained for twenty-five strains of Bacillus spizizenii (ANI 95.01–95.48% and GGDC 62.70–60.00%). The whole-genome phylogenetic relationship showed that SPB7T formed an individual and separated clade with the Bacillus spizizenii group. Principal cellular fatty acids identified in strain SPB7T were anteiso C15:0, anteiso C17:0, iso C15:0, iso C17:0, C16:0, C10:0 3OH and iso C17:1 ϖ10c. Polar lipid profile showed presence of diphosphotidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and five unknown lipids. Cells were rod shaped, catalase, oxidase-positive and motile. Growth occurred at 20–45 °C (optimal 35 °C), at pH 6.0–10.0 (optimal pH 8) and 0–10% (w/v) NaCl (optimal 2%). The phenotypic, biochemical, and genotypic traits of strain SPB7T strongly supported its taxonomic affiliation as a novel species of the Bacillus genus, for which the name Bacillus rugosus sp. nov. is proposed. The type strain is SPB7T (= NRRL B-65559T, = CICC 24827T, = MCC 4185T). |
| Databáze: | OpenAIRE |
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