TrkA and mitogen-activated protein kinase phosphorylation are enhanced in sympathetic neurons lacking functional p75 neurotrophin receptor expression
| Autor: | Sari S. Hannila, Michael D. Kawaja, Gregory M. Ross, Gail Lawrance |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
MAPK/ERK pathway
Time Factors Neurite Blotting Western Cell Count Mice Inbred Strains Receptors Nerve Growth Factor Superior Cervical Ganglion Biology Tropomyosin receptor kinase A Receptor Nerve Growth Factor Mice Nerve Growth Factor Animals Low-affinity nerve growth factor receptor Phosphorylation Receptor trkA Protein kinase A Cells Cultured Mice Knockout Neurons General Neuroscience Membrane Proteins Precipitin Tests Molecular biology Rats Nerve growth factor Animals Newborn nervous system Mitogen-activated protein kinase biology.protein sense organs Mitogen-Activated Protein Kinases Carrier Proteins |
| Zdroj: | European Journal of Neuroscience. 19:2903-2908 |
| ISSN: | 1460-9568 0953-816X |
| DOI: | 10.1111/j.0953-816x.2004.03381.x |
| Popis: | This study examined the effects of hypomorphic p75 neurotrophin receptor (p75NTR) expression and high levels of nerve growth factor (NGF) on trkA phosphorylation and downstream activation of p44/42 mitogen-activated protein kinase (MAPK). Post-ganglionic sympathetic neurons from postnatal day 1 p75NTR exon III null mutant (p75 - / - ) and 129/SvJ mice were cultured in the presence of 50 ng/mL NGF and analysed by Western blotting. Levels of phosphorylated trkA are increased in p75 - / - neurons compared with 129/SvJ neurons, and these higher levels are maintained with continuous exposure to NGF. MAPK is also phosphorylated to a greater extent in p75 - / - neurons than in 129/SvJ neurons, both within 10 min of exposure to NGF, and with continuous NGF treatment for 5 days. These data provide new insight into the mechanism underlying enhanced neurite outgrowth in p75 - / - neurons, demonstrating that trkA and MAPK signalling in sympathetic neurons are increased when p75NTR function is disrupted. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |