Human bone marrow mesenchymal stem/stromal cells produce efficient localization in the brain and enhanced angiogenesis after intra-arterial delivery in rats with cerebral ischemia, but this is not translated to behavioral recovery
| Autor: | Franziska Nitzsche, Matti Korhonen, Kristina Kuptsova, Joanna K. Huttunen, Johanna Nystedt, Jukka Jolkkonen, Bhimashankar Mitkari, Erja Kerkelä |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Stromal cell Time Factors Angiogenesis External carotid artery Ischemia Mesenchymal Stem Cell Transplantation Cell therapy Behavioral Neuroscience Antigens CD medicine.artery medicine Animals Humans Infusions Intra-Arterial Rats Wistar Analysis of Variance business.industry Mesenchymal stem cell Mucin-1 Extremities Infarction Middle Cerebral Artery Mesenchymal Stem Cells Anatomy Recovery of Function medicine.disease Magnetic Resonance Imaging Rats Endothelial stem cell Disease Models Animal business Psychomotor Performance Homing (hematopoietic) |
| Zdroj: | Behavioural brain research. 259 |
| ISSN: | 1872-7549 |
| Popis: | Intravascular cell therapy is a promising approach for the treatment of stroke. However, high accumulation of cells to lungs and other filtering organs is a major concern after intravenous (i.v.) cell transplantation. This can be circumvented by intra-arterial (i.a.) cell infusion, which improves homing of cells to the injured brain. We studied the effect of i.a. delivery of human bone marrow-derived mesenchymal cells (BMMSCs) on behavioral and histological outcome in rats after middle cerebral artery occlusion (MCAO). Sixty male Wistar rats were subjected to transient MCAO (60 min) or sham-operation. BMMSCs (1×10(6)) were infused into the external carotid artery on postoperative day 2 or 7. Histology performed after a 42-day follow-up did not detect any human cells (MAB1281) in the ischemic brain. Endothelial cell staining with RECA-1 revealed a significant increase in the number of blood vessels in the perilesional cortex in MCAO rats treated with cells on postoperative day 7. Behavioral recovery as assessed in three tests, sticky label, cylinder and Montoya's staircase, was not improved by human BMMSCs during the follow-up. In conclusion, human BMMSCs did not improve functional recovery in MCAO rats despite effective initial homing to the ischemic hemisphere and enhanced angiogenesis, when strict behavioral tests not affected by repeated testing and compensation were utilized. |
| Databáze: | OpenAIRE |
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