High-Yield, Magnetic Harvesting of Extracellular Outer-Membrane Vesicles from Escherichia coli
Autor: | Rui Shi, Ziliang Dong, Chongqing Ma, Renfei Wu, Rui Lv, Sidi Liu, Yijin Ren, Zhuang Liu, Henny C. van der Mei, Henk J. Busscher, Jian Liu |
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Přispěvatelé: | Personalized Healthcare Technology (PHT), Man, Biomaterials and Microbes (MBM) |
Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
magnetic nanoparticles
extracellular outer-membrane vesicles PURIFICATION Magnetic Phenomena BIOGENESIS PEGylation VACCINE General Chemistry TRANSPORT Biomaterials Extracellular Vesicles biomimetic method transcriptomes Escherichia coli NANOPARTICLES Humans General Materials Science biofilms GRAM-NEGATIVE BACTERIA Biotechnology |
Zdroj: | Small, 18(48):2204350. WILEY-V C H VERLAG GMBH |
ISSN: | 1613-6810 |
DOI: | 10.1002/smll.202204350 |
Popis: | Extracellular outer-membrane vesicles (OMVs) are attractive for use as drug nanocarriers, because of their high biocompatibility and ability to enter cells. However, widespread use is hampered by low yields. Here, a high-yield method for magnetic harvesting of OMVs from Escherichia coli is described. To this end, E. coli are grown in the presence of magnetic iron-oxide nanoparticles (MNPs). Uptake of MNPs by E. coli is low and does not increase secretion of OMVs. Uptake of MNPs can be enhanced through PEGylation of MNPs. E. coli growth in the presence of PEGylated MNPs increases bacterial MNP-uptake and OMV-secretion, accompanied by upregulation of genes involved in OMV-secretion. OMVs containing MNPs can be magnetically harvested at 60-fold higher yields than achieved by ultracentrifugation. Functionally, magnetically-harvested OMVs and OMVs harvested by ultracentrifugation are both taken-up in similar numbers by bacteria. Uniquely, in an applied magnetic field, magnetically-harvested OMVs with MNPs accumulate over the entire depth of an infectious biofilm. OMVs harvested by ultracentrifugation without MNPs only accumulate near the biofilm surface. In conclusion, PEGylation of MNPs is essential for their uptake in E. coli and yields magnetic OMVs allowing high-yield magnetic-harvesting. Moreover, magnetic OMVs can be magnetically targeted to a cargo delivery site in the human body. |
Databáze: | OpenAIRE |
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