Identification Of Major Adh Genes In Ethanol Metabolism Of Pichia Pastoris
| Autor: | Mehmet Inan, Fidan Erden-Karaoğlan, Mert Karaoglan, Semiramis Yılmaz |
|---|---|
| Přispěvatelé: | EBYÜ, Mühendislik Fakültesi |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Glycerol crabtree-negative yeast Genes Fungal Bioengineering Biology 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Pichia Pichia pastoris ethanol metabolism Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Sequence Analysis Protein 010608 biotechnology Gene Expression Regulation Fungal ethanol production Genetics Escherichia coli Ethanol fuel Ethanol metabolism Promoter Regions Genetic Gene 030304 developmental biology Alcohol dehydrogenase 0303 health sciences Ethanol Sequence Homology Amino Acid Methanol Alcohol Dehydrogenase biology.organism_classification Yeast Recombinant Proteins Alcohol oxidase Culture Media Alcohol Oxidoreductases Glucose chemistry biology.protein Biotechnology |
| Popis: | The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) is a successful host widely used in recombinant protein production. The widespread use of a methanol-regulated alcohol oxidase 1 (AOX1) promoter for recombinant protein production has directed studies particularly about methanol metabolism in this yeast. Although there is comprehensive knowledge about methanol metabolism, there are other mechanisms in P. pastoris that have not been investigated yet, such as ethanol metabolism. The gene responsible for the consumption of ethanol ADH2 (XM_002491337, known as ADH3) was identified and characterized in our previous study. In this study, the ADH genes (XM_002489969, XM_002491163, XM_002493969) in P. pastoris genome were investigated to determine their roles in ethanol production by gene disruption analysis. We report that the ADH900 (XM_002491163) is the main gene responsible for ethanol production in P. pastoris. The ADH2 gene, previously identified as the only gene responsible for ethanol consumption, also plays a minor role in ethanol production in the absence of the ADH900 gene. The investigation of the carbon source regulation mechanism has also revealed that the ADH2 gene exhibit similar expression behaviours with ADH900 on glucose, glycerol, and methanol, however, it is strongly induced by ethanol. |
| Databáze: | OpenAIRE |
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