ChickRH6: a chicken whole-genome radiation hybrid panel

Autor: Florence Plisson-Petit, Chantal Delcros, Martine Yerle, Frédérique Pitel, Philippe Pinton, Mireille Morisson, Alain Vignal, Maxime Galan, Valerie Fillon, Sarah Bosc, Alexandre Lemière, Katia Feve
Přispěvatelé: Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
Jazyk: angličtina
Rok vydání: 2002
Předmět:
Hypoxanthine Phosphoribosyltransferase
carte génétique
Genetic Linkage
[SDV]Life Sciences [q-bio]
Chick Embryo
Genome
HYBRIDES D'IRRADIATION
Cricetinae
Genetics(clinical)
mapping
lcsh:SF1-1100
Genetics
0303 health sciences
CHICKENS
RADIATION HYBRIDS
GENETIC MAPS
Contig
radiation hybrid
Chromosome Mapping
04 agricultural and veterinary sciences
General Medicine
Female
Ploidy
Genetic Markers
lcsh:QH426-470
chicken
poulet
Hybrid Cells
Biology
Cell Line
03 medical and health sciences
Gene mapping
Animals
Radiation hybrid mapping
Genotyping
Gene
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Radiation Hybrid Mapping
Research
0402 animal and dairy science
040201 dairy & animal science
lcsh:Genetics
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
Genetic marker
Animal Science and Zoology
lcsh:Animal culture
Chickens
Zdroj: Genetics Selection Evolution
Genetics Selection Evolution, BioMed Central, 2002, 34 (4), pp.521-533. ⟨10.1051/gse:2002021⟩
Genetics Selection Evolution, Vol 34, Iss 4, Pp 521-533 (2002)
Genetics, Selection, Evolution : GSE
Genetics Selection Evolution 4 (34), 521-533. (2002)
HAL
ISSN: 0999-193X
1297-9686
DOI: 10.1051/gse:2002021⟩
Popis: As a first step towards the development of radiation hybrid maps, we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl2 cells. Due to the low retention frequency of the chicken fragments, a high number of clones was produced from which the best ones were selected. Thus, 452 fusion clones were tested for retention frequencies with a panel of 46 markers. Based on these results, 103 clones with a mean marker retention of 23.8% were selected for large scale culture to produce DNA in sufficient quantities for the genotyping of numerous markers. Retention frequency was tested again with the same 46 markers and the 90 best clones, with a final mean retention frequency of 21.9%, were selected for the final panel. This panel will be a valuable resource for fine mapping of markers and genes in the chicken, and will also help in building BAC contigs.
Databáze: OpenAIRE
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