Quercetin alleviates generalized hyperalgesia in mice with induced adenomyosis
| Autor: | Xishi Liu, Jichan Nie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Pathology Sensory Receptor Cells Uterus TRPV Cation Channels Biology Biochemistry p38 Mitogen-Activated Protein Kinases quercetin 03 medical and health sciences Mice 0302 clinical medicine Dorsal root ganglion Internal medicine Ganglia Spinal Genetics medicine Animals Adenomyosis Phosphorylation Extracellular Signal-Regulated MAP Kinases Molecular Biology mouse Kinase Body Weight Myometrium Articles medicine.disease Immunohistochemistry Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Oncology adenomyosis Hyperalgesia 030220 oncology & carcinogenesis Molecular Medicine Female medicine.symptom Tamoxifen medicine.drug |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Adenomyosis is a common gynecologic disorder characterized by the presence of endometrial glands and stroma within the myometrium. The present study investigated the effect of quercetin in neonatal Imprinting Control Region mice with tamoxifen‑induced adenomyosis. The body weight and hotplate response latency of all mice was examined at 4, 8, 12 and 16 weeks after birth. The mice dosed with tamoxifen were divided into four groups: high‑ or low‑quercetin group, valproic acid (VPA) group and untreated group. The group of mice that were neonatally administrated with the solvent only (no tamoxifen), received no treatment and served as a blank control group. After 3 weeks of drug treatment, the potential ability of quercetin to improve the generalized hyperalgesia in mice with induced adenomyosis was evaluated by determining the body weight, pain modulation, examining the myometrial infiltration by histology examination of the uterus and detecting the expression of transient receptor potential cation channel subfamily V member 1 (Trpv‑1), phospho (p)‑p38 mitogen activated protein kinase p‑extracellular signal‑regulated kinase (p‑ERK) in DRG neurons via immunohistochemistry. The results demonstrated that treatment with quercetin improved the generalized hyperalgesia by extending the hotplate response latency, reduced myometrial infiltration and decreased the expression levels Trpv‑1, p‑p38 and p‑ERK in dorsal root ganglion neurons. The results indicated that quercetin decreases the incidence of hyperalgesia in mice with tamoxifen‑induced adenomyosis, and the potential mechanism is through reduced central sensitization, which may be a promising treatment for adenomyosis. |
| Databáze: | OpenAIRE |
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