Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia
| Autor: | Jane E. Dixon, Hong-Sheng Wang, Wenmei Shi, Ira S. Cohen, David McKinnon, Zongming Pan, Randy S. Wymore |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Complementary Potassium Channels Physiology Molecular Sequence Data Xenopus Gene Expression Biology Xenopus laevis ELK1 Proto-Oncogene Proteins Coding region Gene family Animals RNA Messenger Cloning Molecular Gene ets-Domain Protein Elk-1 Cloning Brain Chemistry Mammals Messenger RNA Ganglia Sympathetic Sequence Homology Amino Acid Rapid Report biology.organism_classification Molecular biology Sciatic Nerve Potassium channel Ether-A-Go-Go Potassium Channels Rats DNA-Binding Proteins Transcription Factors |
| Zdroj: | The Journal of physiology. 511 |
| ISSN: | 0022-3751 |
| Popis: | Three new members of the EAG potassium channel gene family were identified in rat and the complete coding sequence of one of these genes (elk1) was determined by cDNA cloning. The elk1 gene, when expressed in Xenopus oocytes, encodes a slowly activating and slowly deactivating potassium channel. The elk1 gene is expressed in sympathetic ganglia and is also expressed in sciatic nerve. Six of the seven known EAG genes were found to be expressed in rat sympathetic ganglia, suggesting an important functional role for these channels in the sympathetic nervous system. A family of three related voltage-gated potassium channel genes, which are distinct from the Shaker class of potassium channel genes, have been previously identified in either Drosophila or mammals (Warmke & Ganetzky, 1994). The first gene identified in this family was the ether ago-go gene (eag), which was identified initially in Drosophila (Warmke et al. 1991). Two related genes were subsequently described: the eag-related gene (erg) and the eag-like K+ channel gene (elk) (Warmke & Ganetzky, 1994). This family of channel genes is known as the EAG family (Wei et al. 1996). Mammalian homologues of the eag and erg genes have been identified and the potassium channels that they encode have been expressed and characterized (Warmke & Ganetzky, 1994; Ludwig et al. 1994; Robertson et al. 1996; Shi et al. 1997). To date, however, no mammalian homologue of the Drosophilaelk gene has been described, nor have the biophysical properties of any elk channel been characterized. In this paper, three new members of the mammalian EAG gene family are identified. For one of these genes, elk1, we describe the complete coding sequence and have determined the biophysical and pharmacological properties of the encoded channel. In addition, we have determined the mRNA distribution pattern in sympathetic ganglia and sciatic nerve of all known EAG genes. |
| Databáze: | OpenAIRE |
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