Epiblast inducers capture mouse trophectoderm stem cells in vitro and pattern blastoids for implantation in utero
| Autor: | Jinwoo Seong, Javier Frias-Aldeguer, Viktoria Holzmann, Harunobu Kagawa, Giovanni Sestini, Heidar Heidari Khoei, Yvonne Scholte Op Reimer, Maarten Kip, Saurabh J. Pradhan, Lucas Verwegen, Judith Vivié, Linfeng Li, Anna Alemany, Jeroen Korving, Frank Darmis, Alexander van Oudenaarden, Derk ten Berge, Niels Geijsen, Nicolas C. Rivron |
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| Přispěvatelé: | CTR, RS: MERLN - Complex Tissue Regeneration (CTR), Hubrecht Institute for Developmental Biology and Stem Cell Research, Cell biology |
| Rok vydání: | 2022 |
| Předmět: |
Placenta
Stem Cells ACTIVATED PROTEIN-KINASE Cell Biology Trophoblasts/metabolism Trophoblasts EMBRYOS LACKING SELF-RENEWAL TROPHOBLAST Mice FATE DECISIONS Blastocyst Pregnancy POTENTIAL ROLE Genetics Molecular Medicine Animals Female Embryo Implantation CULTURE-CONDITIONS ESSENTIAL REGULATOR Germ Layers GENE-EXPRESSION |
| Zdroj: | Cell Stem Cell, 29(7), 1102-1118.e8. CELL PRESS Cell stem cell, 29(7), 1102-1118.e8. Cell Press Cell Stem Cell, 29(7), 1102-1118.e8. Cell Press Cell Stem Cell |
| ISSN: | 1875-9777 1934-5909 |
| Popis: | The embryo instructs the allocation of cell states to spatially regulate functions. In the blastocyst, patterning of trophoblast (TR) cells ensures successful implantation and placental development. Here, we defined an optimal set of molecules secreted by the epiblast (inducers) that captures in vitro stable, highly self-renewing mouse trophectoderm stem cells (TESCs) resembling the blastocyst stage. When exposed to suboptimal inducers, these stem cells fluctuate to form interconvertible subpopulations with reduced self-renewal and facilitated differentiation, resembling peri-implantation cells, known as TR stem cells (TSCs). TESCs have enhanced capacity to form blastoids that implant more efficiently in utero due to inducers maintaining not only local TR proliferation and self-renewal, but also WNT6/7B secretion that stimulates uterine decidualization. Overall, the epiblast maintains sustained growth and decidualization potential of abutting TR cells, while, as known, distancing imposed by the blastocyst cavity differentiates TR cells for uterus adhesion, thus patterning the essential functions of implantation. |
| Databáze: | OpenAIRE |
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